Insulin receptor substrate-I (IRS-I) is a major cytosolic substrate of the insulin receptor Expression of insulin receptor and IRS-I, and the distribution of these components of the insulin-signalling pathway, were investigated in rat retinae. Insulin receptor and IRS-I were located in retinal sections with anti-insulin receptor and anti-IRS-I antibodies. Reverse transcription-polymerase chain reaction (RT-PCR) of retinal mRNA was performed with primers specific for insulin receptor and IRS-I gene sequences. Immunohistochemistry demonstrated distinct but closely associated staining patterns for insulin receptor and IRS-I throughout rat retinae. The RT-PCR product from rat retinal insulin receptor mRNA corresponded to the high affinity insulin receptor isoform. The RT-PCR product for retinal IRS-I mRNA agreed with that predicted from the gene sequence. The expression of IRS- I and insulin receptors indicates a signalling mechanism by which insulin can influence retinal metabolism or function.

The results of this trial demonstrated that the calculation of IOL power based on ocular axial length measurement with PCI technology provided no clinical advantage over conventional applanation ultrasound, as measured by postoperative refractive outcome (anzctr.org.au number, ACTRN12608000077369).

The data showing increased oxidative damage in baseline GPx-deficient retina give rise to the hypothesis that increased oxidative stress provides a "preconditioning" environment in which protective mechanisms paradoxically render GPx1-deficient retinas less vulnerable to light-induced oxidative damage. This study identified glutaredoxin-2 as a potential candidate.