SummaryThe genetic identification and distribution of Anisakis larvae in Indonesia is described. 110 Auxis rochei rochei and 45 Decapterus russellii were sampled from fish markets in North (Anturan) and South (Kedonganan) Bali. Nematode larvae from A. rochei rochei, Caesio cuning and Epinephelus areolatus from Kedonganan and from Coryphaena hippurus from Pelabuhan Ratu, South Java, were identified using sequence analysis of the internal transcribed spacers (ITS-1, ITS-2) and 5.8S region of rDNA. The larvae belonged to Anisakis typica with an identical sequence to this species from the spinner dolphin (Stenella longirostris) from Brazil, and to 2 further genotypes that differed from that sequence by 0.24 -0.47 %. A. typica occurred in the migratory A. rochei rochei and C. hippurus, while Anisakis sp. 1 and 2 were isolated from the same fish species and the non-migratory C. cuning and E. areolatus. The latter genotype is distinguishable by 4 positions in the ITS-1 region (1.1 %), a genetic distance that indicates the presence of an Indonesian A. typica sibling species. The musculature infection in A. rochei rochei was low (2.5 %), indicating no major risk for the fish consumers. The much higher A. typica infection of fish intermediate hosts in the northern Bali coast is suggested to be dependent on the large dolphin population (nematode final hosts) in the waters off Lovina Beach (North Bali).

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Aim:This study was designed to validate the effectiveness of the pig confinement system (PCS) in reducing the prevalence of zoonotic and internal parasite burdens in pigs.Materials and Methods:Ten PCS households were selected together with 10 households practising traditional scavenging systems. Five pigs were monitored per household every 3 months for 15 months and blood and feces collected. Pigs received a single dose of oxfendazole at 30 mg/kg at baseline. Qualitative fecal examinations for intestinal parasite stages were performed, and serum was tested for antibodies to cysticercus of Taenia solium, Trichinella spp., and Toxoplasma gondii.Results:Based on fecal examination, the prevalence of pigs positive for parasite eggs was reduced in PCS pigs over consecutive samplings (Ascaris suum [14.3% to 0%], Trichuris suis [46.9% to 8.3%], Strongyle-type eggs [81.6% to 8.3%], Physocephalus spp. [6.1% to 0%], and Metastrongylus apri [20.8% to 0%]) compared with increases in the number of pigs positive for parasite eggs in non-PCS pigs (T. suis [20-61.5%], Strongyle-type [60.4-80.8%], Physocephalus spp. [8.3-15.4%], and M. apri [20.8-34.6%]) and little change in pigs positive for A. suum (18.8-19.2%). While the prevalence of pigs with antibodies against to cysticerci of T. solium reduced in PCS pigs from 18% to 14%, the prevalence in non-PCS pigs increased from 42% to 52%. Antibodies to Trichinella were not detected, but the prevalence of T. gondii antibodies increased from 6% to 10% in PCS pigs and from 7% to 24% in non-PCS pigs.Conclusion:These data demonstrate the potential of a PCS to reduce the prevalence of pigs infected with zoonotic and internal parasites and thus the risk to human and pig health.

Pemeliharaan sapi bali secara Sistem PertanianTerintegrasi dapat mengontrol kesehatan ternak serta diharapkan mampu mencegah/menekan serangan penyakit pada ternak sapi. Penelitian ini bertujuan untuk mengetahui prevalensi dan mengidentifikasi jenis protozoa gastrointestinal di Simantri Kecamatan Mengwi. Sampel yang digunakan adalah feses sapi bali yang masih segar berjumlah 105 sampel, yang berasal dari 21 Simantri di Kecamatan Mengwi Kabupaten Badung. Pemeriksaan feses dilakukan dengan menggunakan metode pengapungan gula sheater serta identifikasi protozoa berdasarkan morfologi dan morfometri. Hasil penelitian menunjukkan prevalensi infeksi protozoa gastrointestinal pada sapi bali Simantri Kecamatan Mengwi Kabupaten Badung yaitu sebesar 39,04%. Hasil identifikasi jenis protozoa yang menginfeksi sapi bali antara lain Coccidia sebesar 35,23%, Balantidium sp 7,61%,  dan Entamoeba sp sebesar 2,85%. Tidak terdapat hubungan yang nyata (P>0,05) antara frekuensi kebersihan kandang dengan infeksi protozoa gastrointestinal.

Ektoparasit merupakan parasit yang hidup secara parasitik di bagian luar tubuh hospesnya atau terowongan didalam kulit yang masih berhubungan dengan lingkungan luar. Dampak yang ditimbulkan oleh infestasi ektoparasit pada anjing antara lain penurunan bobot badan, penurunan produksi, kerontokan rambut, iritasi, anemia sampai dengan kematian. Tujuan penelitian ini adalah untuk mengetahui prevalensi infestasi dan identifikasi ektoparasit yang menginfestasi Anjing Kintamani Bali (AKB). Sampel AKB yang digunakan berjumlah 100 ekor. Anjing Kintamani Bali yang dipilih menjadi sampel dinyatakan positif jika ditemukannya ektoparasit pada anjing tersebut. Identifikasi jenis ektoparasit berdasarkan morfologi. Data yang didapatkan dianalisis dengan Uji Chi-square. Hasil penelitian menunjukkan prevalensi ektoparasit pada AKB di Bali didapatkan sebesar 56%. Jenis-jenis ektoparasit yang menginfestasi antara lain: Riphicephalus sanguinus, Ctenocephalides felis, Heterodoxus spiniger dan Trichodectes canis. Uji Chi-square mendapatkan bahwa cara pemeliharaan berhubungan dengan prevalensi infestasi ektoparasit pada AKB di Bali., sedangkan umur dan jenis kelamin tidak berbuhungan. Pemeliharaan Anjing Kintamani Bali di Bali, sebaiknya lebih memperhatikan kesehatan hewan. Memandikan hewan secara rutin sangat disarankan untuk pengendalian infestasi ektoparasit, selain itu pemilik dapat menyisir hewan secara rutin. Sanitasi kandang bagi hewan yang dikandangkan sangat perlu untuk diperhatikan selain itu sebaiknya bagi hewan yang sudah terinfestasi parah dapat dibawa ke dokter hewan terdekat.