Scientific inquiry and project-based learning can foster the ability to think, work, and be scientific and communicate it as an important aspect of Life Skills. Science learning emphasizes the provision of direct learning experience through the use and development of process skills and scientific attitudes, so as to empower the high thinking ability of Elementary School Pre-Service Teacher (ESPT). The aim of this study to determine the competence profile of high-order thinking skills (HOTS) ability of 120 pre-service teachers in science materials at Universitas Sebelas Maret. Sources of data in the form of scores of test results of high order thinking skills that have been validated and tested its reliability. High order thinking skills tests include creative thinking skills and analyzing processes. The technique of data retrieval is done by giving written test instrument in the form of stuffing to the student, then corrected level of truth of answer based on predetermined criteria. The ESPT improvement on creative thinking skills can be seen from the average of N-Gain. The data obtained were analyzed descriptively quantitatively. This study shows that the competence of preservice teachers in HOTS has a mean of 33.2% (40 ESPT). This indicates that the high thinking skills of prospective elementary teachers are medium category, but still needed improvement on certain materials.