Gesneriaceae plant family is comprised of resurrection species, namely Boea hygrometrica and Paraboea rufescens, that are native to the Southeast Asia and Haberlea rhodopensis, Ramonda myconi, and Ramonda serbica, which are mainly found in the Balkan Peninsula. Haberlea rhodopensis is known to be able to survive extreme and prolonged dehydration. Study was carried out after the dried plant Haberlea rhodopensis Friv. had been hydrated and had reached its fresh state. Two juice samples were collected from the plant blossom: The first sample was prepared with 1% filtered water through a patented EVOdrop device. Then the sample was saturated with hydrogen with EVOdrop booster to a concentration of 1.2 ppm, pH = 7.3, ORP = −390 mV. This first sample was prepared with filtered tap water from Sofia, Bulgaria. The second sample, which was a control one, was developed with tap water from Sofia, Bulgaria, consisting of 1% solutions of Haberlea rhodopensis. A study revealed that during the drying process in H. rhodopensis the number of free water molecules decreases, and water dimers are formed. The aim of our study was to determine the number of water molecules in clusters in 1% solutions of hydrated H. rhodopensis plants. Results were analyzed according to the two types of water used in the experiment. Th EVOdrop device is equipped with an ultranano membrane and rotating jet nozzle to create a vortex water and saturation thanks to a second device EVObooster to obtain hydrogen-rich water. In the current study Hydrogen-rich water is referred to as Hydrogen EVOdrop Water (HEW). Research was conducted using the following methods—spectral methods non-equilibrium energy spectrum (NES) and differential non-equilibrium energy spectrum (DNES), mathematical models, and study of the distribution of water molecules in water clusters. In a licensed Eurotest Laboratory, the research of tap water before and after flowing through the EVOdrop device was proven. Studies have been carried out on the structuring of water molecule clusters after change of hydrogen bond energies. The restructuring comes with rearrangement of water molecules by the energy levels of hydrogen bonds. Local extrema can be observed in the spectrum with largest amount of water molecules. The structural changes were tested using the NES and DNES spectral methods. The conducted research proved that the application of EVOdrop device and EVObooster changes the parameters of water to benefit hydration and health.

The method of Non-equilibrium Energy Spectrum (NES) was applied in measurement of hydrogen bonds energy distribution in 1% Rosa damscena L. oil solution in deionized water. Local maxima in this spectrum were identical with these obtained in investigations of other biologically active solutions and related to particular bio effects as follows: (-0.1387 eV; 8.95 µm; 1117 cm-1). This local maximum is typical for antibacterial, anti-tumor and anti-inflammatory effects. The local maxima at (-0.1212 eV; 10.23 µm; 978 cm-1) and (-0.1262 eV; 9.82 µm; 1018 cm-1) are typical for anti-inflammatory effects and this at (-0.1112 eV; 11.15 µm; 897 cm-1) is typical for effects on the nervous system and nerve conductivity. Information theoretical analysis was performed using the values of Shannon entropy and Transformational information entropy, pointing to hydrogen bonds distribution similarities between Rosa damscena L., V. myrtillus L. and Salvia divinorum Epling. The possible chemical causes of these similarities were identified as antioxidant activity and polyphenols concentration.

Spectral analyses of 1% water extracts of fresh and dry Hypericum perforatum L. and 1% dry H. perforatum with colloidal nano silver (NSPs) 30 ppm were conducted. The nano silver is standardised and patented by the Swiss company Evodrop. Non-equliblrium energy spectrum (NES) and Differential non-equliblrium energy spectrum (DNES) methods were used for the spectral analysis. A comparative analysis of 1% extracts of fresh and dry H. perforatum was performed in order to determine the local extremums for effects of nerve tissue conductivity at (-0.1112) eV, anti-inflammatory (-0.1212) eV, anti-tumor effects (-0.1387) eV. The results showed stimulating effect on the nervous system and improvement of nerve conduction (local extremums E=-0.1112 eV)(?=11.15 ?m) (?=897 cm-1), as well as anti-inflammatory effect (E = -0.1212 eV)( ?=10.23 ?m) (?=978 cm-1) and inhibition of development of tumor cells at a molecular level (E=-0.1387 eV) (?=8.95 ?m) (?=1117 cm-1). It was found that clusters of 16 and 15 water molecules are formed in the water herbal extracts of fresh H. perforatum and of dry H. perforatum with AgNPs 30 ppm. The fresh plant showed better results then the dry one. The addition of colloidal nano silver 30 ppm led to better results of the drug.

Drinking water that meets quality requirements is essential for humans. The quality of drinking water is assessed by various indicators and monitored in various ways. Pollution of the hydrosphere leads to the need to use unique water treatment methods since water can absorb various pollutants as a universal solvent. The problem of providing the population with high-quality drinking water is further complicated by the shortage of fresh water. This is due to climate change of technogenic origin and other environmental problems. Environmental monitoring of water quality indicates problems associated with the excess content of several chemical elements in drinking water, which negatively affects its consumer properties. The deterioration of the quality of drinking water due to pollution of the hydrosphere necessitates the development and improvement of water treatment processes, including water filtration. Water that has undergone a water treatment process also requires a study of its parameters. An analysis of publications indicates that a comprehensive study of water quality indicators is difficult. At the present stage, it is necessary to analyze water's physical, chemical, and biological characteristics. From the point of view of the possibility of providing a comprehensive analysis of water quality indicators, the gas-discharge radiation method is promising. Black and white method (Kirlian, 1949, Antonov, Yuskesselieva, 1968, Inyushin et al., 1968, Boyer, Tiller, 1973) or color corona spectral analysis (Ignatov, 2007) is used in medicine, biology, and ecology to study biological and physical objects. Research method (Pesotskaya, Glukhova, Lapitskiy, 2013) refers to active experiments since it is based on registering a corona discharge, which occurs when an object under study is exposed to a high-frequency pulsed electric discharge. The geometric and photometric parameters of the corona gas-discharge radiation depend on the properties of the experimental object of study. Registration of the corona of gas-discharge radiation can be performed by fixing it in grayscale on X-ray film or as a color image on photographic film. The physical effects underlying the method are based on the ionization process, which ensures the formation of a sliding gas discharge formation on the dielectric surface. The discharge occurs due to the formation of ionized gas in the form of a conducting plasma. The dynamics of the formation of a gas discharge in the form of a corona, consisting of separate discharge tracks (streamers), correlates with the electromagnetic properties of a water sample. The geometric shape and surface brightness of gas discharges are determined and recorded on photographic material. The described physical effects make it possible to study the properties of an experimental object.

Drinking water that meets quality requirements is essential for humans. The quality of drinking water is assessed by various indicators and monitored in various ways. Pollution of the hydrosphere leads to the need to use unique water treatment methods since water can absorb various pollutants as a universal solvent. The problem of providing the population with high-quality drinking water is further complicated by the shortage of fresh water. This is due to climate change of technogenic origin and other environmental problems. Environmental monitoring of water quality indicates problems associated with the excess content of several chemical elements in drinking water, which negatively affects its consumer properties. The deterioration of the quality of drinking water due to pollution of the hydrosphere necessitates the development and improvement of water treatment processes, including water filtration. Water that has undergone a water treatment process also requires a study of its parameters. An analysis of publications indicates that a comprehensive study of water quality indicators is difficult. At the present stage, it is necessary to analyze water's physical, chemical, and biological characteristics. From the point of view of the possibility of providing a comprehensive analysis of water quality indicators, the gas-discharge radiation method is promising. Black and white method (Kirlian, 1949, Antonov, Yuskesselieva, 1968, Inyushin et al., 1968, Boyer, Tiller, 1973) or color corona spectral analysis (Ignatov, 2007) is used in medicine, biology, and ecology to study biological and physical objects. Research method (Pesotskaya, Glukhova, Lapitskiy, 2013) refers to active experiments since it is based on registering a corona discharge, which occurs when an object under study is exposed to a high-frequency pulsed electric discharge. The geometric and photometric parameters of the corona gas-discharge radiation depend on the properties of the experimental object of study. Registration of the corona of gas-discharge radiation can be performed by fixing it in grayscale on X-ray film or as a color image on photographic film. The physical effects underlying the method are based on the ionization process, which ensures the formation of a sliding gas discharge formation on the dielectric surface. The discharge occurs due to the formation of ionized gas in the form of a conducting plasma. The dynamics of the formation of a gas discharge in the form of a corona, consisting of separate discharge tracks (streamers), correlates with the electromagnetic properties of a water sample. The geometric shape and surface brightness of gas discharges are determined and recorded on photographic material. The described physical effects make it possible to study the properties of an experimental object.