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Photoactivation of plants by laser treatment is a promising direction in the development of modern agricultural production. Treatment of plants with radiation with specified characteristics stimulates the development of plants, the formation of generative traits and an increase in yield. An approach based on the use of a specialized laser installation mounted on an unmanned aerial vehicle (UAV) is proposed to automate the process of photoactivation of large cultivated areas. It is possible to perform laser activation of large areas with minimal expenditure of time and human resources due to autonomous processing of the field with the help of UAVs. An algorithm for calculating a covering trajectory for covering large rectangular areas with a laser spot with given characteristics is proposed in the paper. A methodology for calculating the required power of the laser installation depending on the altitude and flight time of the UAV is presented. The advantage of the developed approach is its versatility, since this approach takes into account the characteristics of a laser installation and can be used with devices of various types. Depending on the laser parameters, the algorithm builds such a trajectory for the UAV so that the irradiation of plant seedlings is uniform throughout the entire processing process. Field experiments were conducted when the UAV moved along the calculated trajectory at a speed of 0.3 m/s and the average processing time for a field 200 m long and 1 m wide was 9 minutes. The results of field experiments show that laser irradiation on most of the studied crops increased the yield and height of the stand (in cereals - in four out of six crops, in legumes - in four out of five studied crops). The proposed algorithm for constructing a path for uniform laser irradiation of a site takes into account the area of the laser spot to ensure the required radiation characteristics when using any laser installation.