<p>Kanker serviks merupakan kanker yang paling sering dijumpai pada wanita setelah kanker payudara. Kanker ini terkait dengan infeksi persisten virus yaitu Human Papillomovirus (HPV). Virus ini mengekspresikan protein onkogenik virus yaitu protein E6 dan E7 yang terkait dengan proses karsinogenesis. Salah satu mekanisme onkogenik virus ini adalah pengikatan protein p53 yang menginduksi degradasi oleh protein E6, mengakibatkan efek anti-apoptosis dan proliferasi sel secara terus menerus. Minyak cengkeh yang mengandung senyawa aktif eugenol telah dilaporkan memiliki efek anti kanker pada beberapa kanker. Namun, mekanisme minyak cengkeh yang terkait dengan penghambatan kanker servik masih belum jelas. Penelitian ini, untuk mengkaji efek minyak cengkeh dari Syzygium aromaticum dalam efek pro-apoptosis sel kanker servik HeLa terkait dengan kadar protein p53. Tingkat apoptosis diamati dengan pewarnaan dengan Annexin-V dan PI dan dilakukan dengan metode flow-cytometry. Dilakukan pengecatan immunohistokima untuk melihat ekspresi caspase-3 aktif untuk mengonfirmasi sel yang apoptosis, sedangkan kadar protein p53 dievaluasi dari lisat sel menggunakan ELISA protein p53. Hasil studi menunjukkan bahwa minyak cengkeh memiliki efek pro-apoptosis, berkaitan dengan kadar protein p53, pada sel kanker serviks secara in vitro.</p>

Purpose: To evaluate the anti-cancer activity of Indonesian Pasuruan propolis extract (PPE) against HT-29 human colon carcinoma cell line. Methods: HT-29 cells were cultured and treated in different concentrations of PPE (50, 100, 200, 400 µg/mL) for 24 h. The cells were evaluated by several indicators such as cell proliferation and apoptosis as well as the expression of protein Ki67, p53, cyclin D1, and Bcl-xL. Results: Administration of PPE inhibited cell proliferation of HT-29 in concentration-dependent manner but the effect was not significant (p = 0.842); the results were similar with regard to the expression of Ki67 in HT-29 cells (p = 0.953). Administration of 400 µg/mL PPE insignificantly decreased cyclin D1 expression (p = 0.149). The concentration of PPE at 50, 100, and 200 µg/mL induced cell apoptosis of HT-29 cells initially, but the level of apoptosis subsequently decreased (p = 0.416). Furthermore, the expressions of p53 and Bcl-xL decreased following treatment with PPE at 50, 100 and 200 µg/mL but increased for the PPE 400 µg/mL group (p = 0.000). Conclusion: PPE reduced the expressions of p53, Ki67, cyclin D1, and Bcl-xL insignificantly as it it generally failed inhibit cell proliferation and promote cell apoptosis of HT-29 cells.

Background: Nasopharyngeal carcinoma (NPC) is an endemic malignancy in Asia and in Indonesia with a high incidence and mortality. The high mortality rate of patients with NPC is caused by recurrence and metastases early in the disease process, even though they have been given a combination of standard NPC therapy, namely radiotherapy and chemotherapy. Recurrences generally occur after the initial modalities of radiotherapy. Toxicity due to therapy given over a long time can also increase mortality. This study aims to determine the expression of BCL11B in undifferentiated NPC and its correlation with LMP-1 so that it can provide an overview of the nature of CSC in NPC, which is thought to cause recurrence and metastases and provide a poor prognosis in patients with NPC. Methods: This study was an analytical observational study using a cross-sectional approach using 30 samples from nasopharyngeal biopsy tissue diagnosed with undifferentiated NPC at the anatomical pathology installation of Dr. Saiful Anwar General Hospital, Malang, Indonesia, between 2018-2020. LMP-1 and BCL11B expression was examined using the immunohistochemical method. Data analysis was performed univariate and bivariate with the help of SPSS software.   Results: Statistical analysis using the Spearman correlation test between LMP-1 and BCL11B expression in undifferentiated NPC biopsy tissue showed a significant correlation (p=0.004) with a correlation coefficient of r=-0.511. Conclusion: The higher the expression of LMP-1, the lower the expression of BCL11B in nasopharyngeal biopsies of patients with undifferentiated.

ABSTRAKMultiple Myeloma (MM) adalah keganasan sel plasma yang termasuk golongan Malignant Small Round Cell Tumor ditandai oleh ekspansi immunoglobulin monoklonal dan akumulasi abnormal sel plasma di dalam kompartemen sum-sum tulang. Kami laporkan kasus MM non sekretorik yang jarang ditemui yaitu pasien laki-laki 54 tahun yang datang dengan keluhan benjolan pada paha kiri dan benjolan pada bahu kanan dengan hipogammaglobulinemia dan protein Bence Jones negatif. Hasil pemeriksaan klinis, radiologi, dan biopsi menunjukkan hasil sesuai MM dengan diagnosis banding metastase karsinoma dan Non Hodgkin's Lymphoma. Hasil pulasan immunohistokimia CD 20 (-), sitokeratin () dan LCA (-) menyingkirkan diagnosis banding dan dikonfirmasi dengan hasil biopsi sumsum tulang yaitu suatu plasmasitomatosis. Pasien didiagnosis dengan multiple myeloma non sekretorik. Dari kasus ini dapat disimpulkan bahwa pemeriksaan klinis dan penunjang sangat diperlukan untuk menegakkan diagnosis MM non sekretorik dengan tepat dan kombinasi beberapa pemeriksaan immunohistokimia dapat membantu penegakan diagnosa. LCA (-) Kata Kunci: Hipogammaglobulinemia, Multiple Myeloma non sekretorik ABSTRACT Multiple Myeloma (MM) is malignancies of plasma cells that belong to Malignant Small Round Cell Tumor characterized by immunoglobulin monoclonal expansion and accumulation of abnormal plasma cells in the bone marrow compartment. A rare case of non-secretory MM was found on a 54 year male patient who came with a bone tumor in the left thigh and on the right shoulder with hypogammaglobulinemia and negative Bence Jones protein. The results of clinical examination, radiology, and biopsy showed the results matched to MM with differential diagnosis of carcinoma metastase and Non Hodgkin's Lymphoma. The results of immunohistochemical CD 20 (-), cytokeratin (-) and

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