Mueller Hinton Agar merupakan media yang direkomendasikan oleh World Health Organization (WHO) untuk uji kepekaan bakteri terhadap antibiotika sedangkan Media Tahu secara umum dapat digunakan sebagai basal seperti halnya agar nutrient untuk menyiapkan media kaya dengan penambahan suplemen tertentu misalnya darah. Keduanya merupakan media padat, non selektif dan mempunyai kemiripan susunan makanan. Penelitian ini bertujuan menentukan perbedaan hasil uji kepekaan Staphylococcus aureus dan Escherichia coli menggunakan Mueller Hinton Agar dan Media Tahu. Penelitian uji kepekaan menggunakan Mueller Hinton Agar dan Media Tahu dengan metode difusi Kirby-Bauer, dimana masing-masing bakteri diuji terhadap 5 jenis antibiotika. Staphylococcus aureus diuji terhadap Trimethoprim-sulfametoxazole, Tetracycline, Clindamycin, Vancomycin, dan Erythromycin sedangkan Escherichia coli diuji terhadap Ampicillin, Tobramycin, Amoxiclav, Ciprofloxacin, dan Nalidixic Acid. Suspensi bakteri dengan konsentrasi sesuai standard Mac Farland 0,5 ditanam pada Mueller Hinton Agar dan Media Tahu, lalu diletakkan disk-antibiotika. Zona hambatan terlihat sebagai zona jernih di sekitar disk-antibiotika. Hasil penelitian memperilhatkan daya hambat antibiotika TE 30 µg, MEM 10 µg, dan OX 5 µg terhadap S.aureus dalam media tahu lebih besar dibandingkan MHA, sedangkan antibiotika SXT 25 µg dan E 15 µg dya hambatnya lebih kecil. Daya hambat antibiotika AMP 10 µg dan AML 25 µg terhadap E.coli dalam media tahu lebih besar dibandingkan MHA, untuk C 30 µg sama besar, sedangkan TOB 10 µg dan CIP 5 µg lebih kecil, Untuk. Kesimpulan penelitian menunjukkan bahwa media Tahu dapat digunakan sebagai pengganti MHA pada uji kepekaan S.aureus dan E.coli.
Banana trees are plants that come from the humid tropics and include herbaceous plants from Southeast Asia, including Indonesian. Several types of bananas are scattered in various parts of Indonesia, namely Ambon bananas, Ambon moss bananas, Barangan bananas, Emas bananas, Kepok bananas, Lampung bananas, jackfruit bananas, horn bananas, and Raja Bulu bananas.One of the food ingredients that can be processed into processed products that have commercial value is bananas. Processing of bananas consists of two processing groups, that is: processing of unripe bananas and processing of ripe (ripe) bananas. One of the processing of ripe bananas can be done by fermentation. The end products of fermentation include energy, carbon dioxide, ethanol, ammonia, lactic acid, and acetic acid. Normally, acetic acid can be consumed after ripe bananas have been fermented for at least 3 days. Making acetic acid with bananas as raw material is called banana vinegar. This study aimed to observe differences in the levels of acetic acid (CH3COOH) in Ambon bananas fermented for 7, 10, 14, and 21 days. The results of the study on average levels of acetic acid in Ambon bananas fermented for 7, 10, 14 and 21 days were 0.59%, 0.68%, 0.77% and 0.84%. And from the results of statistic (p = 0.062 at the 95% confidence level (p > 0.05)).
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