Objective: This study represents the first attempt to investigate the antimicrobial activity of Thuja occidentalis seeds extracts against the isolated skin infecting microorganisms. Methods:T. occidentalis seeds were powdered and extracted continuously by Soxhlet apparatus using 96% ethanol to obtain the total crude ethanolic extract. The extracts with increasing polarity were successively prepared with petroleum ether, chloroform, ethyl acetate, and methanol using the Soxhlet apparatus.The extracts were screened for their phytochemical constituents. Their antimicrobial activity against standards microorganism and the isolated skin infecting microorganisms was evaluated using the agar diffusion method. Results:The result revealed the presence of flavonoids, alkaloids, terpenoids, tannins and cardiac glycosides. The antimicrobial activity result showed that Escherichia coli, Pseudomonas aeroginosa, Staphylococcus aureus, Bacillus subtilis and Candida albicans were found to be sensitive to the extracts of T. occidentalis seeds with inhibition zones range between 20±0.57-12±1.25 mm. The isolated skin infecting microorganisms; Staphylococcus aureus and Staphylococcus epidermidis were found to be highly susceptible to the extracts of T. occidentalis seeds with inhibition zones of 28±1.02-22±0.45 mm and minimum inhibitory concentration (MIC) values of 6.25 mg/ml. Conclusion:From the results it concludes, T. occidentalis seeds extracts had a considerable level of antimicrobial activity. They are more effective towards gram-positive than gram-negative bacteria. Hence, the active principles present in the extracts are particularly powerful effective against the skin infected isolated bacteria. In conclusion, there is a proportional increasing in the antimicrobial activity with increase of the solvents polarity. This may attribute to the fact that the chemical constituents responsible for the antimicrobial activity are of high polarity, such as tannins.

Objective: The content and chemical composition of dry distillates of the stem, stem wood and stem bark of Acacia seyal were investigated. The distillates are fumigants of A. seyal locally known in Sudan as Dokhan and widely used for its cosmetic, aromatic and medicinal value for the treatment of candidiasis, genital yeast infection, urinary tract infection, diarrhoea, respiratory tract infection, skin infection and with potent, antioxidant and antimicrobial activities. Methods:The dry distillates were prepared by dry distillation method from the Acacia seyal stem, stem wood and stem bark and investigated chemically by GC-MS analysis. Results:The percentage yield of dry distillates (Dokhan) wer found to be 4.0 %, 4.8%, 1.4.1% v/w for stem, stem wood and stem bark respectively. GC-MS analysis revealed the presence of one hundred and twenty three constituents in the stem distillate with major constituents of solerone (7.27%), furfural (7.15%), catechol (7.11%), syringol (5.56%), allo-inositol (4.86%), mequinol (4.81%), furfuralcohol (3.35%), 3-methyl-1,2-cyclopentanedione (3.24%), phenol (2.73%), homovanillyl alcohol (2.56%) and 3-cresol (2.11%). the wood distillate show detection of eighty compounds, with main compounds: 1, 3-dimethyl-5-methoxypyrazol (10.61%), syringol (6.75%), furfuralcohol (5.24%), mequinol (4.49%), 1,2-anhydro-3,4,5,6-alloinositol (4.26%), 3-methyl-1,2-cyclopentanedione (3.42%), catechol (3.37%), 3-methoxycatechol (3.22%), homovanillyl alcohol (2.78%), homosyringic acid (2.40%), 3-cresol (2.18%), 3-methyl-2-cyclopentenone (2.44%) and 1,2-cyclopentanedione (2.03%). Sixty six compounds were detected in the distillate bark and the main compounds were found to be hexadecanoic (62.83%), catechol (3.38%), tetrapentacontane (3.18%), phenol (2.72%), mequinol (2.30%) and 2-ethylfurane (2.22%). Conclusion:The result concludes that the medicinal Sudanese Dokhan have good potential as sources of different bioactive compounds and antioxidants.

Objective: This study represents the first attempt to investigate the antimicrobial activity of Peganum harmala, and Trachyspermum ammi seeds extract against the isolated bacillary dysentery-causing microorganisms.Methods: T. ammi and P. harmala were extracted by 96% ethanol using Soxhlet apparatus. The extracts were screened for their phytochemical constituents. Their antimicrobial activity against the isolated dysentery-causing microorganisms was evaluated using the agar diffusion method.Results: The antimicrobial activity result showed that, the two isolated bacteria, Shigella flexneri, and Shigella dysenteriae were found to be sensitive to the extract of T. ammi seed with inhibition zones up to 25 mm, compared to the inhibition zone of 20 mm produced by Gentamycin standard drug, this is mainly due to the presence of the different phytochemical in the extract such as tannin, flavonoids, terpenoids which are well known for their antimicrobial effects. The two isolated bacteria were found to be insensitive (zero mm) to P. harmala extract, Amoxicillin, and Amoclan (Amoxicillin+clavulanic acid) standard drugs, this is due to the fact that, the phytochemicals constituents of P. harmala possess the antagonistic effect to each other’s. Addition to; these bacteria became resistant to both Amoxicillin and Amoclan.Conclusion: From the results it concludes, T. ammi seeds extract had a considerable level of antimicrobial activity against bacillary dysentery-causing microorganisms resistant to Amoxicillin and Amoxicillin+clavulanic acid drugs.

Objective: The current study compares the anticoagulant activity of curcumin and ginger rhizome extracts in vitro. Background: Curcuma longa and Zingiber officinale (Ginger) (turmeric, curcuma, or curcumin) Rhizomes are widely used as a spice and in herbal medicine around the world. Methods: To obtain the entire crude extract, Zingiber officinale and Curcuma longa rhizomes were pulverized and extracted in 70% ethanol. The phytochemical contents of the extracts were determined. Their anticoagulant impact was tested in vitro on blood samples from healthy Sudanese people by assessing prothrombin time (PT) and activated partial thromboplastin time (APTT). Results: The ginger extract inhibited coagulation and significantly prolonged prothrombin time (PT) in a dose-dependent manner (25,50, 75 μl) with P. value 0.001, whereas APTT showed insignificant prolongation (P. value 0.139). PT and APTT of curcumin showed insignificant prolongation among (25, 50, 75μl) with P. value 0.55 and 0.146, respectively. Conclusion: The in vitro anticoagulant effect demonstrated that ginger and curcumin extracts had equal anticoagulant activity, but curcumin has a higher anticoagulant impact with considerable PT prolongation. Keywords: Zingiber officinale, Curcuma longa, Coagulation, PT, APTT.