No abstract

SummaryThe rgp7 gene, originally isolated from rice seedlings, encodes a small GTP-binding protein which is related to the product of the human proto-oncogene, ras-p21. To determine the physiological role of the rgpl protein, rgpl-p25, the coding region of rgpl was introduced into tobacco plants in both sense and antisense orientations. Transformants, which were found to contain the rgpi gene at up to three loci, showed distinct phenotypic changes. The most notable was a reduction in apical dominance with increased tillering, together with dwarfism or abnormal flower development or both. These effects were similarly observed in both sense and antisense transformants. Northern hybridization analysis showed that rgp7 was expressed only in phenotypically abnormal transformants and not in the apparently normal phenotypes. Furthermore, the R1 progenies from most transformants co-segregated into a 31 ratio for both kanamycin resistance and tillering. The expression of tgp7, a presumed tobacco homologue of rgpl, was markedly reduced in transformants expressing the antisense rgpi, whereas it was apparently unaffected in transformants with sense rgpl. These observations suggest that the phenotypic changes in antisense transformants may be mediated by an effect on native tgp7 mRNA, whereas in sense transformants the changes may be induced by over-production of rgplp25. The possibility that the increased tillering may be related to abnormal phytohormone metabolism or response pathways, and that rgpl -p25 may mediate the transmission of signals in these pathways is discussed.

In this study, more than 90% of the Japanese Jr(a-) phenotypes had c.376C>T (Gln126Stop) nucleotide change. In the other Jr(a-), a new mutation (c.2T>C) in the start codon encoding Thr instead of Met, c.1515delC encoding Ala505AlafsStop and heterozygous for c.337C/T and c.736C/T were detected. DNA-based genotyping is accurate and useful for Jr(a-) donor typing.

No abstract

By programming the A102 ABO variant into the system software with the new allele combinations, the BLOODchip(®) Reference is a suitable genotyping tool to be applied to Asian samples.