Palabras clave: Cuerpo de agua dulce, Eutrofización, Cianobacterias tóxicas, Cylindrospermopsis sp., Microcystis sp. ENSAMBLAJE FITOPLANCTÓNICO DE LA LAGUNA DE CAJITITLÁN, JALISCO DURANTE EL AÑO 2015 ENERO-JUNIO/AÑO 7/2020/NÚMERO 13/5-15

No abstract

Macroalgae contains high concentrations of polysaccharides, polyphenols and secondary metabolites. Those compounds are factors that prevent the isolation of deoxyribonucleic acid (DNA) and therefore inhibit the polymerase chain reaction (PCR) that is the beginning for the application of any molecular marker. In the present study, the application of six extraction methods was documented; four of them conventional and two commercial kits. The highest efficiency in DNA extraction was obtained with a conventional method with modifications. Said modifications consisted of immersing the algal tissue in ß-mercantoethanol and the addition of the DIECA salt in the extraction buffer. To test the purity of the DNA, in addition to the electrophoresis and spectrophotometry methods, a PCR was performed for the ISSR molecular marker, obtaining amplified fragments using the aforementioned modification.

The Jalisco coast presents a great heterogeneity of environments that provide the necessary conditions for the establishment anddevelopment of macroalgae; These environments are described in the present study, relating them to the diversity of macroalgaerecorded and collected in 19 locations on the Jalisco coast from 2000 to 2018. The results show that the very particular andheterogeneous characteristics and conditions in each of the locations were determinants for the richness and distribution of speciesto be different along the coast of Jalisco. The locality with the highest species richness was Chamela, with 105 registered species,being the rocky substrate where most of the species were found, regardless of the time of year (rainy and dry season). Through aclassification analysis, based on the presence/absence of species, and a non-metric multidimensional scaling (NMDS), threegeographical zones were recognized on the Jalisco coast: north, center and south, this being the one that registered the highestnumber of species. (193).