Scabies transmission caused by the female mite Sarcoptes scabei var. hominis, can occur due to direct or indirect contact with the patient's skin. Risk factors that cause scabies were socio-economic, patient age, gender, personal hygiene, environmental sanitation, culture, and Islamic boarding schools management. This study aims to determine the relationship between personal hygiene and environmental sanitation with the scabies incidence and differences in personal hygiene and environmental sanitation between men and women in Islamic boarding schools. This type of research was quantitative observational using an analytic survey study with a cross-sectional approach with 114 sample. Analysis used frequency distribution and bivariate analysis using the Chi Square test and the Mann-Whitney Test with a p-value less than 0.05. The results showed that there was a significant relationship between personal hygiene subvariables: skin cleanliness, antiseptics use, cleanliness of clothing and prayer equipment, towels cleanliness, hand and nail hygiene, bed cleanliness, and genital hygiene, with the scabies incidence. Environmental sanitation sub-variables include lighting and water cleanliness, which are significantly related to the scabies incidence. Also, there are significant differences in personal hygiene and environmental sanitation between men and women which can affect the scabies incidence  at the Asy-Syadzili 4 Gondanglegi Islamic boarding school, Malang. Abstrak: Penularan skabies yang disebabkan oleh tungau betina Sarcoptes scabei var. hominis, dapat terjadi karena kontak langsung atau tidak langsung dengan kulit penderita. Faktor risiko penyebab skabies yaitu sosial ekonomi, usia penderita, jenis kelamin, personal hygiene, sanitasi lingkungan, budaya, dan manajemen pondok pesantren. Penelitian ini bertujuan mengetahui hubungan personal hygiene dan sanitasi lingkungan dengan kejadian skabies serta perbedaan personal hygiene dan sanitasi lingkungan antara laki-laki dengan perempuan di pondok pesantren. Jenis penelitian adalah kuantitatif observasional menggunakan studi survei analitik dengan pendekatan cross sectional dengan total sampel 114. Analisis menggunakan analisis distribusi frekuensi dan bivariat menggunakan uji Chi Square dan Mann-Whitney Test dengan p-value kurang dari 0,05. Hasil penelitian menunjukkan bahwa terdapat hubungan yang signifikan antara subvariabel personal hygiene yang meliputi: kebersihan kulit, penggunaan antiseptik, kebersihan pakaian dan alat solat, kebersihan handuk, kebersihan tangan dan kuku, kebersihan tempat tidur, dan kebersihan genitalia, dengan kejadian skabies. Sub-variabel sanitasi lingkungan meliputi pencahayaan dan kebersihan air, berhubungan secara signifikan dengan kejadian skabies. Serta terdapat perbedaan yang signifikan personal hygiene dan sanitasi lingkungan antara laki-laki dengan perempuan yang bisa mempengaruhi kejadian skabies di pondok pesantren Asy-Syadzili 4 Gondanglegi Malang.