Lactobacillus plantarum Dad-13 is a probiotic candidate from dadih, a traditional fermented buffalo milk from West Sumatera, Indonesia. This study aimed to evaluate safety aspects of L. plantarum Dad-13 in high dose consumption on Sprague Dawley rats. Two treatment groups were fed with 10 11 CFU/mL/day of L. plantarum Dad-13 for 14 and 28 days respectively. Two placebo groups were fed with 1 mL skimmed milk per day for 14 and 28 days and a fifth, untreated group used as control. Feed intake and body weight were monitored, while blood samples and mesenteric lymph node (MPN) organs were dissected. Organ weight, leukocyte profiles, glutamic-oxaloacetic transaminase (GOT) activity, plasma malondialdehyde (MDA) concentration and intestinal morphology were measured. Microbial analyses were conducted on fecal matter, digesta, blood and organs. Results showed that consumption did not negatively affect general health, organ weight, leukocyte profiles, GOT activity, MDA concentration and intestinal morphology. Numbers of L. plantarum in treated rats' feces were significantly increased, indicating its survival in gastrointestinal tracts. Bacteria in the blood and organs of both groups were identified using rep-PCR with BOX A1R primer, which revealed that it was not identical to L. plantarum Dad-13. Thus, L. plantarum Dad-13 did not translocate in the organs and blood of rats. Therefore, L. plantarum Dad-13 is suggested as likely to be safe for human consumption.

Pemerintah Republik Indonesia telah menetapkan bahwa seluruh produk yang beredar di Indonesia haruslah terjamin kehalalannya, peraturan tersebut terdapat pada Undang-Undang No.33 Tahun 2014, dan telah dikawal persiapan pelaksanaanya oleh Badan Penyelenggara Jaminan Produk Halal (BPJPH). Untuk itu diperlukan sosialisai dalam rangka persiapan sertifikasi halal tersebut terutama produk cemaran babi dan turunannya pada warung makan yang berada di sekitar kecamatan Kartasura, Sukoharjo, Jawa Tengah. Sosialisasi dilaksanakan dengan metode pemberian materi, tanya jawab, dan bertukar pengalaman, kemudian peningkatan pengetahuan diketahui dengan metode pre-test dan post-test pada 10 orang perwakilan tiap warung makan. Metode analisis data dalam penelitian ini adalah metode penelitian kuantitatif untuk mengetahui peningkatan pengetahuan peserta yang diukur dengan menggunakan pre-test dan post-test dan diuji statistic menggunakan paired sample t-test. Hasil yang didapatkan pada pengabdian masyarakat ini adalah meningkatnya pengetahuan peserta pada produk yang mengandung cemaran babi dan turunannya, sehingga para pemilik warung makan tersebut dapat mewaspadai adanya cemaran babi pada bahan baku produk yang akan mereka jual.The Republic of Indonesia's Government has determined that all products circulating in Indonesia must be guaranteed halal. This regulation is contained in Law No.33 of 2014, and the preparation for implementation has been guarded by the Halal Product Guarantee Agency (BPJPH). Thus, socialization is needed to prepare for halal certification, significantly pork contaminated products, and their derivatives at food stalls around Kartasura sub-district, Sukoharjo, Central Java. The socialization was carried out by using the method of providing material, question, and answer, and exchanging experiences, then increasing knowledge was known by the pre-test and post-test methods to 10 representatives of each food stall. The data analysis method in this study is a quantitative research method to determine the increase in participants' knowledge which is measured using pre-test and post-test and tested statistically using paired sample t-test. The results obtained from this community service are the increased knowledge of participants on products containing pork contaminants and their derivatives so that the owners of these food stalls can be aware of pork contamination in the raw materials for their products to sell.

No abstract

Penelitian ini bertujuan untuk mengetahui bagaimana intensitasmenonton televisi dan minat baca siswa di masa pandemi COVID-19 sertaapakah terdapat hubungan antara intensitas menonton televisi dengan minatbaca siswa di masa pandemi COVID-19. Jenis penelitian yang digunakan yaitukuantitatif korelasional. Penelitian ini dilakukan di MI Al Manar Sambirejomulai dari Desember 2020-Mei 2021. Populasi dalam penelitian ini yaituseluruh siswa kelas V MI Al Manar Sambirejo sebanyak 46 siswa dengan sampel42 siswa. Pengambilan sampel menggunakan teknik sampel acak sederhana.Teknik pengumpulan data diperoleh dari angket dan dokumentasi. Hasilpenelitian menunjukkan bahwa 1) Intensitas menonton televisi siswa kelas V dimasa pandemi COVID-19 dalam kategori durasi tinggi yaitu antara 3-4 jam/hari2) Minat baca siswa kelas V di masa pandemi COVID-19 dalam kategori sedangyaitu mayoritas siswa jarang menggunakan waktu luang untuk membaca buku3) Berdasarkan uji korelasi product moment diperoleh nilai signifikansi sebesar0,000 < 0,05 dan nilai r hitung -0,705 > r tabel 0,304 artinya h0 ditolak dan h1diterima, dapat disimpulkan bahwa terdapat hubungan negatif antaraintensitas menonton televisi dengan minat baca siswa kelas V pada masaPandemi COVID-19 di MI Al Manar Sambirejo Tahun 2020/2021. Hubungannegatif menunjukkan semakin tinggi intensitas menonton TV maka semakinrendah minat baca

No abstract