Propolis merupakan salah satu produk potensial yang dapat dihasilkan dari olahan sarang lebah madu hutan. Selama ini, petani madu di Desa Batu Dulang, Kabupaten Sumbawa hanya mengambil madu hutan dengan cara ditiriskan dan disaring langsung dari sarangnya. Setelah itu, sarang lebah tidak dimanfaatkan dengan optimal. Padahal, pada sarang lebah madu tersebut terdapat propolis yang kaya akan bioflavonoid.Tujuan dari penelitian ini adalah untuk menghasilkan propolis dari sarang lebah madu hutan Sumbawa Apis dorsata dan mengidentifikasi senyawa bioflavonoid yang terkandung di dalamnya. Metode yang dilakukan adalah metode eksperimen yang terdiri atas dua tahap. Tahap pertama adalah ekstraksi propolis dari sarang lebah madu Apis dorsata dengan menggunakan metode maserasi dan pemanasan gelombang mikro (microwave assisted extraction). Setelah ekstrak propolis diperoleh, tahap penelitian selanjutnya adalah mengidentifikasi senyawa bioflavonoid yang terkandung menggunakan metode spektrofotometer UV-Vis. Analisis yang dilakukan adalah analisis rendemen dan total bioflavonoid. Pada laporan kemajuan ini, propolis berhasil diekstrak dari sarang lebah madu hutan Apis dorsata, dari Desa Batu Dulang, Kabupaten Sumbawa. Rata-rata rendemen yang dihasilkan adalah 24.43% untuk perlakuan konsentrasi dan pemanasan microwave. Dari tiga konsentrasi pelarut propilen glikol yang digunakan, konsentrasi 20% merupakan konsentrasi pelarut yang optimal dalam menghasilkan rendemen propolis ekstrak pada penelitian ini, yaitu mencapai 30%. Senyawa bioflavonoid dari ketiga konsentrasi akan diuji pada tahap selanjutnya dari penelitian ini.
Senna occidentalis (Caesalpiniaceae) is a pan tropical shrub plant widely distributed in warm regions of the world. The root infusion of the plant has been used for the treatment of gastro-intestinal disorders including emesis. The current research is aimed at evaluating the antiemetic property of the aqueous ethanol extract and solvent fractions of the root and also verifying the classes of phytochemicals in the extract and fractions. The LD 50 of the extract and its solvent fractions was determined. The antiemetic property was evaluated using copper sulphate induced emesis in chicks while phytochemical screening was carried out using standard methods. The three doses of the extract (300, 150 and 75mg/kg) conferred dose against retching compared to the control group. At a dose of 150mg/kg, the extract completely (100%) prevented co promethazine at 150mg/kg, the standard antiemetic used. Other doses of the extract 300 and 75mg/kg showed lower effect relatively. Two of the three fractions of the ethanol extract; Hexane and n retching in a similar degree (100%) demonstrated by the crude ethanol extract and the standard antiemetic promethazine at 150mg/kg, while ethyl acetate fraction exhibited its maximum protection (100%) at the lowest dose of its solvent fractions were found to be non oral route. Identified phytochemicals in the ethanol extract include; unsaturated steroids, cardiac glycosides, saponins and tannins. distributed across different fractions of the extract in order of their polarity; Steroids and resins in the n-hexane fraction, Cardiac glycosides, unsaturated sterols, saponins and tannins in the Ethylacetate and N ethyl acetate fraction contains flavonoids. The observed antiemetic activity of the extract and fractions could be due to one or some of these phytochemicals present.
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