Identification of Total Bioflavonoid Compound of Propolis Extract from Wild Honey Bee Hives Apis dorsata in Sumbawa Region, Indonesia

Rodiahwati, Wawat; Ariskanopitasari, Ariskanopitasari; Saleh, Imam K

doi:10.14416/j.ijast.2019.01.007

Cited by 8 publications

(9 citation statements)

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“…Therefore, it can be concluded that despite the vast biodiversity and the variety of species, bees, in general, will prefer a group of plants that allow them to produce propolis, possibly due to their bioactivity and their ability to generate a sample with higher resistance to mechanical forces. It is well known that various species of the Trifolium genus and, most importantly, Coffea arabica specials are some of the species identi ed in the literature as the main contributors of exudates for different tropical propolis samples [15].…”

Section: Resultsmentioning

confidence: 99%

Physicochemical Profile of Non-polar Extracts of Colombian Propolis (Npcs) and Their Correlation With the Antimicrobial Activity of Pathogenic Strains

Márquez,

Ovalle,

Maraschin

et al. 2023

Preprint

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Honeybees collect multiple types of propolis with high concentrations of bioactive compounds with notorious antimicrobial activity against gram-positive and gram-negative bacteria, undesirable microorganisms in food products. Propolis is produced by collecting and transforming plant exudates around hives. Their composition and action against pathogenic microorganisms vary according to the hive structure and the plant's chemical composition. In the literature, there is a lack of information on the Colombian propolis action against pathogens, and there is no information about the chemical profile of the compounds in these samples. Therefore, to identify propolis's chemical profile and correlate the metabolites with the antimicrobial action, it is necessary to perform a metabolomic analysis that integrates spectroscopic techniques and bioassays. Techniques such as GC-FID, NMR, and U-HPLC were used to obtain the profile and concentration of low molecular weight metabolites, amino acids, fatty acids, and polyphenols. Then, the extracts were analysed by minimum inhibitory concentration (MIC) tests to elucidate the bactericidal power of the extracts. It was concluded that the ecosystem and botanical species are the main characteristics that allow the discrimination of propolis. Additionally, it was seen that the bioactivity against pathogens was due to a synergistic effect between various metabolites, which have variations in their chemical structure, mainly in molecular weight and functional groups. Additionally, it was seen that the ecosystem has a more significant influence on the propolis composition compared to the bee species that collect the exudates.

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Section: Resultsmentioning

confidence: 99%

Physicochemical Profile of Non-polar Extracts of Colombian Propolis (Npcs) and Their Correlation With the Antimicrobial Activity of Pathogenic Strains

Márquez,

Ovalle,

Maraschin

et al. 2023

Preprint

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“…Hydroxycinnamic acids from ground propolis were extracted using purified water and aqueous solutions of selected solubilizers at a sample-to-solvent ratio of 1:20 (w/v). The solubilizers were selected according to published scientific research data in order to improve hydroxycinnamic acid solubility: propylene glycol [12], 2-hydroxypropyl-β-cyclodextrin [17] and sodium bicarbonate [18]. Produced aqueous propolis extracts were filtered using polyvinylidene difluoride (PVDF) membrane filter Frisenette ApS, Q-Max, PVDF, 25 mm, 0.22 µm.…”

Section: Preparation Of Aqueous Propolis Extractsmentioning

confidence: 99%

“…Extraction with selected solubilizers was performed in the same way as it was described previously on the saturation procedure of aqueous propolis extracts. Solubilizers were selected according to scientific data and their aqueous solutions of selected solubilizers were: 5%, 10% and 20% of propylene glycol [12], 5%, 10% and 20% of 2-hydroxypropyl-β-cyclodextrin [17] and 1% and 5% of sodium bicarbonate [18]. Due to sodium bicarbonate reactiveness (quick temperature rise and rapid CO 2 release) during the extraction process, the extraction duration was reduced to 2 min.…”

Section: Preparation Of Aqueous Propolis Extractsmentioning

confidence: 99%

“…For example, Rodiahwati W., with colleagues, attempted to enhance the solubility of propolis bioactive compounds by adding propylene glycol solution and applying microwave-assisted extraction. They demonstrated that using a 20% propylene glycol solution and adding microwave-assisted extraction had the biggest effect on the extraction yield [12].…”

Section: Introductionmentioning

confidence: 99%

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Preparation of Aqueous Propolis Extracts Applying Microwave-Assisted Extraction

2022

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Water-based propolis extracts usually contain up to 10-fold lower quantities of active ingredients due to poor solubility in water of propolis bioactive compounds when compared with ethanol-based extracts. Since ethanol-based extracts are of limited use, water-based extracts are preferred nowadays. The application of alternative extraction techniques should be evaluated to improve extraction efficiency. Aqueous propolis extracts were prepared using purified water and propylene glycol, 2-hydroxypropyl-beta-cyclodextrin and sodium bicarbonate aqueous solutions. A microwave-assisted extraction method was applied in cycles. The total concentration of hydroxycinnamic acids in aqueous propolis extract produced by four extraction cycles was determined to be 1502.1 ± 130.1 μg/mL and 20% propylene glycol, 10% 2-hydroxypropyl-beta-cyclodextrin and 5% sodium bicarbonate aqueous solutions, increasing the total concentration of hydroxycinnamic acids by 1.6, 1.7 and 1.9-fold, respectively. An application of microwave-assisted extraction method and the procedure of repeating extraction cycles reliably increased the quantity of hydroxycinnamic acids in aqueous propolis extracts. Similarly, the presence of propylene glycol, 2-hydroxypropyl-β-cyclodextrin and sodium bicarbonate increased the concentration of the hydroxycinnamic acids in propolis extracts.

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“…asiatica has been reviewed in several studies to consist of various chemical compositions with biological benefits i.e. to symptom treatment, to wound and injury treatment and to mentality treatments [3][4], C. asiatica is abundant in phenolic and flavonoid compounds, which are the key chemicals with antioxidant properties to protect human health against attack of free radical compounds [5][6][7]. Terpenes compounds including monoterpenes, sesquiterpene, and triterpenoids, especially asiaticoside and asiatica acid are the major bioactive compounds existing in C. asiatica extracts.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activities ofCentella asiaticaExtract-loaded Bovine Serum Albumin Nanoparticles in Simulated Gastrointestinal System Study

et al. 2020

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Bioavailability of active compounds extracted from herbs is generally limited to be adsorbed or expressed to target organisms due to several physical and chemical factors. Nanoparticle encapsulation techniques was developed to carry bioactive macromolecules of Centella asiatica (Buabok) in the form of C. asiatica Extract-loaded Bovine Serum Albumin Nanoparticles (CBNPs) to improve bioavailability. In this study, the antioxidant activities of CBNPs and C. asiatica crude extract were evaluated by using DPPH radical scavenging assay in the simulated gastrointestinal system, including mastication, stomach, duodenum, and ileum conditions to provide the environment which similar to in vivo system in terms of chemical and physical parameters. CBNPs were prepared by mixing of C. asiatica crude extract and BSA at different ratio of 1:2, 1:3, and 1:4. The results showed that the highest antioxidant activity of CBNP was observed when the ratio of crude extract and BSA at 1:2 was used at pH 2.0 or in the simulated stomach condition. The denaturation or unfolding of BSA in the simulated stomach occurred when pH was low could lead to the release of active compounds at certain area in gastrointestinal system.

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Identification of Total Bioflavonoid Compound of Propolis Extract from Wild Honey Bee Hives Apis dorsata in Sumbawa Region, Indonesia

Cited by 8 publications

References 0 publications

Physicochemical Profile of Non-polar Extracts of Colombian Propolis (Npcs) and Their Correlation With the Antimicrobial Activity of Pathogenic Strains

Physicochemical Profile of Non-polar Extracts of Colombian Propolis (Npcs) and Their Correlation With the Antimicrobial Activity of Pathogenic Strains

Preparation of Aqueous Propolis Extracts Applying Microwave-Assisted Extraction

Antioxidant Activities ofCentella asiaticaExtract-loaded Bovine Serum Albumin Nanoparticles in Simulated Gastrointestinal System Study

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