Introduction Acne vulgaris is a multifactorial disease. Recent study showed that inflammation does have a central role in the formation of both inflammatory and noninflammatory lesions in acne vulgaris. There are various findings of proinflammatory cytokines related to acne vulgaris, but no previous study correlate interleukin- (IL-) 19 to acne vulgaris. This pilot study aims to look at difference in IL-19 serum concentration on degrees of severity of acne vulgaris. Methods This is an analytical observational cross-sectional study. Sample subjects were patients with acne vulgaris who met the inclusion criteria. Enzyme-linked immunosorbent assay (ELISA) study was applied to measure IL-19 serum. Result Analysis test found statistically significant difference between IL-19 serum concentration of group of patients with mild acne vulgaris and that of group of patients with severe acne vulgaris. Moreover, analysis revealed significant difference between IL-19 serum concentration of group of patients with moderate acne vulgaris and that of group of patients with severe acne vulgaris. Conclusions There are differences in serum levels of IL-19 on the severity of acne vulgaris. The significant difference might show that inflammation has a core role in severity of acne vulgaris, and IL-19 might potentially be related to acne vulgaris.

Objective: To discover the ideal concentration of Advanced Platelet Rich Fibrin (A-PRF) as modification of PRF, for human Dental Pulp Stem Cells (hDPSCs) differentiation. Material and Methods: hDPSCs were devided into five experimental groups: Group I (control group) consist of hDPSCs cultured in 10% FBS, Group II consist of hDPSCs cultured in 1% A-PRF, Group III consist of hDPSCs cultured in 5% A-PRF, Group IV consist of hDPSCs cultured in 10% A-PRF and Group V consist of hDPSCs cultured in 25% A-APRF. All group have been observed for 7 and 14 days and each group had three biological replicates (triplo). Formation of the mineralized nodules was detected after 7 days by Alizarin red-based assay and Dentin Sialophosphoprotein (DSPP) expression after 7 and 14 days quantified by ELISA reader. Statistical analysis was proven with Kruskal-Wallis and post hoc Mann-Whitney test. Results: The differentiation of hDPSCs in all A-PRF groups was significantly different on day-7 (p<0.05) compare to control group (Group I). There were no significant differences between all groups on day-14 (p>0.05). Significantly differences between Group II (1% A-PRF) and Group I (control), Group II (1% A-PRF) and Group III (5% A-PRF), also Group II (1% A-PRF) and Group V (25% A-PRF) was found from post hoc test analysis. Conclusion: The ideal conditioned media concentration for differentiation of human dental pulp stem cells was on 1% up to 5% A-PRF group.

Senescent human dermal fibroblasts had reduced capacity in proliferation and collagen synthesis. It is due to unresponsiveness against transforming growth factor-β1 (TGF-β1) stimulation. Either platelet-rich fibrin (PRF)-lysate or hyaluronic acid (HA) can restore TGF-β1 signaling pathway. To determine whether HA addition to PRF lysate has a better activity than PRF-lysate alone in restoring senescent human dermal fibroblasts (HDFs) activities. HDF isolated from six different human skins was divided into normal HDFs and senescent HDFs which are induced by serum starvation. The senescent groups were then given 50% PRF-lysate and various levels of HA. Amelioration of TGF-β1 signaling was measured by cellular proliferation index and collagen deposition.  Addition of HA into PRF-lysate resulted in a significant increase in proliferation index and collagen deposition index than PRF-lysate alone. The best level of HA for this mixture ranged from 20.83 mM to 41.67 mM. HA in PRF lysate is an excellent candidate material for treating clinical signs related to senescent human dermal fibroblasts.   Ethical permission: This experiment had gain approval from the local ethical committee, Ref: KE/FK/471/EC/2016 dated 17-05-2016.

Non-cultured epidermal cell suspension (NCECS) is a relatively new cellular grafting technique for vitiligo. Platelet rich fibrin (PRF) is a platelet and immune concentrate gather on a single fibrin membrane which can be used in conjunction with grafts and has several advantages, such as promoting wound healing, haemostasis, and give better handling properties to graft materials. This study was conducted to determine the efficacy of NCECS combined with PRF in patients with stable vitiligo. Seven patients with stable vitiligo which not responding to topical and phototherapy for more than 12 months were included in the study. The melanocytes were harvested as an autologous melanocyte rich suspension from a donor skin. The non cultured melanocyte transplanted to recipient area that had been superficially dermabraded and smeared with PRF gel. Of all 7 patients, 1 patients showed excellent pigmentation (90-100%), 2 had good repigmentation (60-89%), 1 had fair repigmentation (25-59%) and 3 patients had a poor response (0-24%). The procedure is safe and promising surgical modality for stable vitiligo.

Objective Platelet-rich plasma (PRP) activation is an important factor in triggering the initial release of blood-derived growth factors from platelets. Vascular endothelial growth factor-A (VEGF-A) can be expressed by human dental pulp stem cells (hDPSCs) and plays an important role in dental pulp angiogenesis. The aim of this study is to analyze the effects of calcium gluconate on PRP activation in hDPSC VEGF-A expression. Materials and Methods Two types of PRP and their corresponding activators were analyzed in this study: PRP (activated using calcium chloride/CaCl2) and PRP-T (activated using CaCl2 with the addition of 10% calcium gluconate). hDPSCs were obtained by using an out-growth method (DPSCs-OG), and harvest between the fifth and sixth passages, then cultured in three different media groups: control, PRP, and PRP-T, which were planted in 96 wells (5 × 103 each well). The VEGF-A expression of hDPSCs was analyzed by using an ELISA test and observed at 24, 48, and 72 hours. Statistical Analysis This study was performed by using one-way ANOVA (p < 0.05) test. Results There were significant differences between all groups (p < 0.05) at 48 and 72 hours of observations, and no significant differences in the PRP and PRP-T groups at 48 and 72 hours of observations (p > 0.05). Conclusion PRP and PRP-T were equally effective in inducing VEGF-A expression of hDPSCs.