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Abstract:Objective: to test the extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.) As dye substitution in Gram staining technique. Material and Methods: Made Preparations from the suspension Escherichia coli ATCC 25922 and Streptococcus sanguine ATCC 10556 counted 30 preparation, and then done Gram staining process to this preparation. 1 preparation using counterstain basic fuchsin as a control and 29 other preparations using ethyl acetate extract of Red Fruit as a counterstain, the result of staining compared to using standard degradation of RHS color chart. Result: From 29 sample preparations used the counterstain extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.), 12 preparations produced a color close to color code 62B on the standard degradation of RHS color chart that is moderate purplish pink. Conclusions: From this preliminary study that examined the extract of ethyl acetate Red Fruit as a counterstain on Gram staining resulted in a color contrast between Gram + and Grambacteria though not as good as if using compressed/counterstain basic fuchsin.

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Inflammation is a body tissue reaction to the damage caused by foreign object. One of the plants that the community uses to eliminate inflammation is cashew leaf (Anacardium occidentale L). This research aimed to find out if the guava extract has an anti-inflammatory effects compared with diclofenac. This was an experimental study using 30-tails Wistar strain, which is divided into 6 groups that every 1 hour later induced lambda-carrageenan. Group I and IV are given ethanolic extract of cashew leaf 300 mg/kg BW. Group II and V were given cellulose (CMC) 1% as a control. Group III and VI were given diclofenac 4.5 mg/kg of BW. Edema volume from hour 1 to 6 and MDA levels of foot tissue in the hour 6 were measured in group I, II, III. Hyperalgesia was measured in group IV, V and VI. All research results were statistically tested with ANOVA test followed by the Newman Keuls test. Ethanolic extract of cashew leaf 300 mg/kg BW inhibited the formation of edema volume, preventing hyperalgesia significantly (P < 0.05) compared to a group of control and diclofenac. Measurement of MDA levels between groups with ethanolic extract of cashew leaf, control, and diclofenac was not significantly different (P > 0.05). Ethanolic extract of cashew leaf has an anti-inflammatory effect based on inhibition of edema volume, hyperalgesia.