Manganese (Mn) is used as main materials in various chemical processes of industry, but it suggested that Mn brings about its toxicant by fume or dust through respiratory system and skin barrier. Mn toxicant induces the loss of mental health and life quality by cerebrovascular and skin diseases. Nevertheless, it lefts much unknown on the mechanism and the effectively therapeutic methods about Mn toxicant. Therefore, this study was evaluated the cytotoxicity induced by manganese dioxide (MnO 2) in cultured NIH3T3 fibroblasts, and also, the correlation between MnO 2-induced cytotoxicity and oxidative stress was examined. While, the effect of Eclipta prostrata L. (EP) extract belong to Compositae was assessed against MnO 2-induced cytotoxicity in the view of antioxidative effect for searching the natural resources mitigating or preventing the MnO 2-induced cytotoxicity. In this study, MnO 2-induced cytotoxicity was revealed as mid-toxic by Borenfreud and Puerner's toxic criteria, and catalase (CAT), an antioxidant prevented MnO 2-induced cytotoxicity by the remarkable increase of cell viability in these cultures. While, in the protective effect of EP extract on MnO 2-induced cytotoxicity, EP extract effectively prevented the cytotoxicity induced by MnO 2 via antioxidative effects such as xanthine oxidase (XO) inhibitory ability and DPPH-radical scavenging ability. From the above results, EP extract showed the effective prevention against MnO 2-induced cytotoxicity correlated with oxidative stress by antioxidative effects. Conclusively, this study may be useful to research or development the alternatively therapeutic agent from natural resources like EP extract for the treatment of diseases resulted in oxidative stress.

This study was performed to evaluate the cytotoxicity of aluminum chloride (AlCl3), and the protective effect of Lonicerae flos (LF) extract on AlCl3-induced cytotoxicity in the cultured C6 glioma cells. Here, the cell viability and antioxidative effects, such as DPPH-radical scavenging activity, superoxide anion scavenging activity, and lipid peroxidation (LP), were assessed. AlCl3 significantly decreased the cell viability in a dose-dependent manner; the XTT50 value was measured at 128.8 M of AlCl3. The cytotoxicity of AlCl3 was determined as highly toxic the y Borenfreund and Puerner's toxic criteria. The butylated hydroxytoluene (BHT) and antioxidant both significantly increased the cell viability, which was damaged by AlCl3-induced cytotoxicity in these cultures. In the protective effect of LF extract on AlCl3-induced cytotoxicity, the LF extract significantly increased the superoxide anion scavenging activity and inhibitory activity of LP, as well as the DPPH-radical scavenging activity. From these results, it is suggested that the oxidative stress may have been involved in the cytotoxicity of AlCl3, and LP extract effectively protected AlCl3-induced cytotoxicity through the antioxidative effects. Conclusively, the natural resources, like LP extract, may be a putative therapeutic agent for the treatment of dementia induced by allergen like aluminum correlated with the oxidative stress.

No abstract

No abstract

No abstract