The first outbreak of African Swine Fever (ASF) in Romania was recorded in a backyard holding in Satu Mare County in July 2017 as a result of a previous large-scale spread of the virus in wild boars and domestic pig populations in two of Romania’s neighbouring countries, Ukraine and the Republic of Moldova. Since then, more than 1.3 million domestic pigs have been infected in more than 5600 backyard holdings and commercial farms all over Romania where stamping out procedures have been performed afterwards. The spread of the disease could not be contained despite the contingency plans developed by the authorities based on the current EU and Romanian legislation, and currently, the entire territory of Romania is considered to be affected, leading to a significant decline of the pig inventory and major damages in the commercial farming system. Consequently, the amounts of pork imported from other EU member states have increased yearly, pig meat becoming the main commodity on the list of agricultural goods purchased by Romania. 2021 has been by far the worst year for Romanian pig meat production, generating the highest financial losses mainly due to the inability of authorities and policymakers to implement effective and efficient disease control measures.

The improvement of the reproductive traits of animals is of great interest for livestock production. Due to its positive impact on the sheep industry’s profitability, prolificacy is one of the most economically significant biological traits, showing variation between and within breeds of domestic sheep (Ovis aries). Different mutations in BMPR-1B, BMP15 and GDF9 genes coding for the transforming growth factor-β (TGFβ) superfamily have been shown to influence the ovulation rate and litter size. Numerous single-nucleotide polymorphisms (SNPs) in the bone morphogenetic protein 15 (BMP15) gene have been linked to ewe fecundity. Using targeted PCR amplification and Sanger sequencing, we were able to identify heterozygous SNPs in exon 2 of BMP15 in three sheep breeds reared in Romania: Tsigai, Cluj Merino and Tsurcana. The sequence analysis revealed three previously documented mutations, namely the missense mutation c.755T>C (L252P), which is predicted to change the tertiary structure of the BMP15 protein, and two silent mutations, c.747T>C (P249P) and c.1047G>A (V349V). In addition, we also identified one novel silent mutation, c.825G>A (S275S). Based on our findings and publicly available data, we indicate four putative mutational hotspots within exon 2 of BMP15 that could be considered for improving the indigenous sheep breeds through targeted gene editing and SNP genotyping strategies.