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The communication describes a theoretical framework for tangent hyperbolic fluid of nano-biofilm due to an extending or shrinking sheet that comprises a stagnation point flow, chemical reaction with activation energy, and bioconvection of gyrotactic microorganisms. The varying transport features due to dynamic viscosity, thermal conductivity, nano-particle mass permeability and microbe organisms diffusivity are taken into account for the novelty of this work. The inspiration is developed to enhance heat transfer. A set of leading partial differential equations is formed along with appropriate boundary constraints. Using similarity transformations, the basic formulation is transitioned into non-linear differential equations. To produce observational data, the shooting technique and Runge-Kutta fourth order method are employed. The coding of numerical scheme is developed in Matlab script. The visual representation of the effects of diverse fluid transport properties and distinctive parameters on speed, temperature, concentration and motile density are evaluated. The velocity become faster when the parameters $$\omega $$ ω , $$\lambda $$ λ , $$\epsilon $$ ϵ and $$V_0$$ V 0 are enhanced. Brownian motion, thermal conductivity, heat generation as well as thermophoresis factors all strengthen the temperature distribution, however the nano-particle concentration profile is enhanced as the nano-particle mass conductivity variable, activation energy as well as the thermophoresis variable are boosted. The microorganism density improves significantly when the microorganism diffusivity factor increases. The skin friction, Sherwood number, Nusselt number and motile density number decline against the incremented transport parameters.