Abstract. The object of study were human teeth with caries cavities and healthy teeth extracted for orthopaedic indications. The main method of study was Raman spectroscopy. We revealed the specific features of Raman spectra for the healthy tooth tissues impaired ones and the tissues affected by caries. The optical criteria are established that allow cares detection and assessment, as well as the detection of precaries impairment of the tooth tissues. The results were analysed in comparison with the data of the chemical analysis performed using a raster electron microscope.

Excessive postoperative scarring halts the effectiveness of glaucoma surgery and still remains a challenging problem. The purpose of this study was to develop a PLA-PEG-based drug delivery system with cyclosporine A or everolimus for wound healing modulation. Methods: PLA-PEG implants saturation with cyclosporine A or everolimus as well as their further in vitro release were analyzed. Anti-proliferative activity and cytotoxicity of the immunosuppressants were studied in vitro using human Tenon’s fibroblasts. Thirty-six rabbits underwent glaucoma filtration surgery with the application of sham implants or samples saturated with cyclosporine A or everolimus. The follow-up period was six months. A morphological study of the surgery area was also performed at seven days, one, and six months post-op. Results: PLA-PEG implants revealed a satisfactory ability to cumulate either cyclosporine A or everolimus. The most continuous period of cyclosporine A and everolimus desorption was 7 and 13 days, respectively. Immunosuppressants demonstrated marked anti-proliferative effect regarding human Tenon’s fibroblasts without signs of cytotoxicity at concentrations provided by the implants. Application of PLA-PEG implants saturated with immunosuppressants improved in vivo glaucoma surgery outcomes. Conclusions: Prolonged delivery of either cyclosporine A or everolimus by means of PLA-PEG implants represents a promising strategy of wound healing modulation in glaucoma filtration surgery.

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Purpose to study laser irradiation inside the eye. Methods we performed simulation and analysis of the physical effects of YAG laser during laser capsulotomy on the enucleated eyes of mature chinchilla rabbits by pulses fixation with photodetectors and evaluation of them on an oscilloscope. Results our study shows that the effect of the laser pulse on the eye membranes can be measured with a high-speed photodetector, that the results are stable and reproducible, the obtained pulses have the same configuration at all recording points. Conclusion the pulse amplitudes behind the posterior capsule of the lens and at the retina, when the pulse is applied to the posterior capsule, have no statistical difference, this can be explained by diffuse scattering of laser radiation inside the eye by the inner surface of the eye. Using our experimental model, it is possible to calculate the peak of the pulse reaching the retina and use this data in further experiments and clinical practice.