(1) Background: this study aims to test the cytotoxicity of three antimicrobial products used in periodontitis treatment on gingival mesenchymal stem cells (gMSCs) and their influence on root surfaces and gMSC adhesion. We tested the null hypothesis that the effects of the antimicrobials did not differ. (2) Methods: the commercial products based on sulphonic/sulphuric acids, sodium hypochlorite and silver nanoparticles, in five different concentrations, were added to culture medium for growing gMSCs. Cell proliferation capacity was tested using the Cell Counting Kit-8 (CCK8) and their viability was determined by succinate dehydrogenase activity (MTT) assay. Scanning electron microscopy evaluated the adhesion of gMSCs on root samples treated mechanically and with commercial products. (3) Results: the products induced a dose-dependent cytotoxicity in terms of reduced proliferation and viability of gMSCs, as well as cell shape modifications. Significant differences in CCK8 values between the different commercial products were observed. Based on proliferation tests, the null hypothesis was rejected. When MTT values of the three products were compared with each other, no significant differences were observed for any of the five concentrations (p = 0.065, p = 0.067, p = 0.172, p = 0.256, p = 0.060). (4) Conclusions: the three antimicrobials had a certain degree of cytotoxicity on gMSCs. gMSCs repopulated treated root surfaces.

Analgesics are used to reduce pain, which is the main symptom experienced in dental inflammatory pathologies. Painkillers are among the most used drugs in dentistry, along with anti-inflammatories and antibiotics. A lack of knowledge regarding well-defined prescription protocols has been observed. The aim of this study was to revise and update the prescription of anti-inflammatories and analgesics according to the latest guidelines. We performed electronic research of selected databases: PubMed, Google Scholar, AAPD, from 2010-2020, in order to identify the most frequent anti-inflammatories and analgesics used in paediatric dentistry. Approximately 25% of all adverse drug reactions are the consequences of treatments with anti-inflammatory drugs. This paper focuses on both the correct and wrong prescription of medication in paediatric dentistry, as well as on the short and long-term side-effects of this prescription.

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The oral pathologies in paediatric patients frequently require the recommendation of adjuvant medication. A lack of knowledge regarding well-defined prescription protocols has been observed in every day practice. The aim of this study was to revise and update the prescription of antibiotics according to the latest guidelines. We performed electronic research of selected databases: PubMed, Google Scholar, AAPD, from 2010-2020 in order to identify the most frequent antibiotics used in paediatric dentistry. The antibiotic resistance of bacteria, as well as the side effects that follow the prescription of antibiotics are reported to be in a continuous growth as a result of inadequate use. Prescription of medication, especially antibiotics in paediatric dentistry, must analyze both short and long-term side-effects.

Periodontitis is a chronic multifactorial polymicrobial infection, characterized by profound modifications of the composition and proportion of the subgingival microbiota. Microbiological laboratory tests are sometimes used in periodontal diagnosis and monitoring of treatment, but both conventional cultivation methods and molecular techniques have some major drawbacks. Therefore, other performant bacterial identification methods must be considered. The aim of the current study was to use Matrix-assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALTI-TOF MS) analysis in association with bacterial culture method to evaluate the modifications of the subgingival bacterial composition in periodontitis patients, before and after cause-related subgingival therapy. Subgingival plaque samples were collected from periodontal pockets before and after subgingival mechanical instrumentation and adjunctive local antimicrobial applications and were cultured in aerobic and anaerobic conditions. Microbial colonies were further assessed using MALDI-TOF-MS. A total of 36 bacterial strains were isolated from a group of 16 patients. All species from the orange complex were identified by MALDI-TOF MS. A marked reduction of detection frequency was observed in most bacterial strains, including the orange complex after cause-related periodontal treatment. The results of this study indicate that MALDI-TOF MS could be considered an accurate method for oral microbial identification and the cause-related periodontal treatment is useful for reducing the microbial burden.