The rise of cycle tourism is based on the use of non-motorized itineraries, which favours a new tourist typology that makes possible the revitalization of rural and peripheral areas. At present, studies of sustainable mobility consider, to a large extent, intermodality, especially among public transportation means, which are not very polluting and collective, as in the case of railways. Andalusia has a huge variety of non-motorized itineraries (livestock trails, rural roads, greenways ...) associated with areas of great scenic and heritage value. The main objective of this research is the establishment of a methodology that allows to identify the existing links between railway lines in use and these itineraries. To do this, at the methodological level, a proximity study was carried out first using the Buffer Geoprocessing tool, and subsequently a Network Analysis to measure accessibility through this extension, in both cases of the ArcGIS program, applied to the Geographic Information System that has been implemented. Its main results are related to the determination of favourable routes for potential users of the "train+bike" intermodality in Andalusia. In addition, its conclusions are, therefore, indicative for further studies of detail.