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The aim of this study was to evaluate the amount and quality of water in the Magdalena-Eslava river system and to propose alternatives for sustainable water use. The system is the last urban river in the vicinity of Mexico City that supplies surface water to the urban area. Historical flow data were analyzed (1973–2010), along with the physicochemical and bacteriological attributes, documenting the evolution of these variables over the course of five years (2008–2012) in both dry and rainy seasons. The analyses show that the flow regime has been significantly altered. The physicochemical variables show significant differences between the natural area, where the river originates, and the urban area, where the river receives untreated wastewater. Nutrient and conductivity concentrations in the river were equivalent to domestic wastewater. Fecal pollution indicators and various pathogens were present in elevated densities, demonstrating a threat to the population living near the river. Estimates of the value of the water lost as a result of mixing clean and contaminated water are presented. This urban river should be rehabilitated as a sustainability practice, and if possible, these efforts should be replicated in other areas. Because of the public health issues and in view of the population exposure where the river flows through the city, the river should be improved aesthetically and should be treated to allow its ecosystem services to recover. This river represents an iconic case for Mexico City because it connects the natural and urban areas in a socio-ecological system that can potentially provide clean water for human consumption. Contaminated water could be treated and reused for irrigation in one of the green areas of the city. Wastewater treatment plants and the operation of the existing purification plants are urgent priorities that could lead to better, more sustainable water use practices in Mexico City.

Helicobacter pylori is a pathogen bacteria associated with chronic gastritis, peptic ulceration, and gastric carcinoma. H. pylori has a spiral morphology, which under certain conditions of stress becomes a coccoid form. This type of morphology has been linked to a viable but non-culturable (VBNC) state, which is thought to allow its persistence in the environment. Membrane damage in VBNC H. pylori in water as a mechanism for inactivation using ozone (O) and chlorine disinfection has not been reported in the literature. In this paper, disinfection assays with ozone and chlorine were conducted to evaluate their effects on VBNC H. pylori cells. The use of fluorescent dyes such as propidium monoazide (PMA) coupled with quantitative real-time polymerase chain reactions produced results necessary to assess the viability of the microorganism and demonstrate the effect of each disinfectant on the bacterial count. Applying ozone showed a 5-log bacterial reduction using a disinfectant concentration and exposure time (CT) of 4 mg min/L. Chlorine disinfection for the same 5-log reduction required a higher CT value. Field emission scanning electron microscope images of ozone-treated VBNC H. pylori also showed severe cell damage. The use of PMA revealed that chlorine produced physical damage in the membrane in addition to the known inhibiting effect on cell enzymatic processes. These findings are important for the detection and control of VBNC H. pylori cells in drinking water systems.

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