The primary diagnoses associated with their infertility included: diminished ovarian reserve (n¼9), male factor infertility (n¼3), endometriosis (n¼3), hypothalamic amenorrhea (n¼2), FSH receptor defect (n¼1), Grave's disease (n¼1), advanced maternal age (n¼1), unexplained infertility (n¼7), and PCOS (n¼17). Two patients were excluded due to insufficient RNA on endometrial biopsy. Chi-square analysis demonstrated patients with PCOS have a twofold (OR¼2.03; p¼0.29) increased likelihood of non-receptive endometrial lining by ERA analysis as compared to those without a diagnosis of PCOS.CONCLUSIONS: Though not statistically significant, the results of the study are clinically relevant as our investigation demonstrates that the odds of having non-receptive endometrial lining for patients with PCOS is at least two times that of patients who are not affected by PCOS. Further investigation on this subject is warranted as an early ERA may decrease the incidence of recurrent implantation failure in PCOS patients.IMPACT STATEMENT: Based on our findings, physicians may have a lower threshold to offer ERA to PCOS patients compared to other infertility diagnoses.

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OHdG) levels were estimated at base line (day 0), 1 month, 3 months and 6 months duration. ROS, DFI, 8-OHdG were also assessed in fathers of healthy children. RESULTS: Semen parameters were assessed as per the WHO, 2010 guidelines. The seminal mean ROS levels (p<0.05), sperm DFI (p<0.001), 8-OHdG (p<0.01) levels were significantly higher in fathers of children with non-familial sporadic heritable retinoblastoma as compared to fathers of healthy children. There was a reduction in mean DFI levels post yoga-based lifestyle intervention at 1 month (p¼0.63), 3 months (p¼0.046) and 6 months (p¼0.032). Similarly, there was a reduction in seminal mean ROS levels at 1 month (p¼0.74), 3 months (p¼0.039) and 6 months (p¼0.006). The levels of DNA oxidative base adduct i.e., 8-OHdG were also significantly reduced at 1 month (p¼0.68), 3 months (p¼0.043) and 6 months (p¼0.022) with respect to its base line levels (day 0). CONCLUSIONS: Yoga-based simple lifestyle intervention meditation may significantly lower oxidative stress and oxidative DNA damage, and levels of mutagenic base 8-OHdG in the sperm DNA. Thus, yoga-based intervention is alternative and complementary treatment for maintaining/restoring sperm DNA integrity and hence, can reduce the prevalence of childhood cancers where poor sperm DNA integrity could be one of the reason.

Relative risk and Odds ratios using standard procedure as well as the Cochran-Mantel-Haenszel test to account for confounding factors were calculated. A value of P<0.05 was considered significant.RESULTS: No differences were denoted in normal fertilization rates and in both the number of usable and expanded blastocysts obtained at day-6 of development between the two Groups [505 out of 736 (62.1%); 42 out of 505 (8.5%) and 25 out of 505 (5.2%)] vs.[501 out of 737(68.6%); 37 out of 501 (8.4%) and 33 out of 501(3.7%)] with p values of 0.8680, 0.8580 and 0.4333,respectively. Significantly improved rates for usable and expanded blastocysts were found for Group 1 at Day-5 of embryo development [216 out of 505 (46.4%) and 103 out of 505 (19.5%)] vs [196 out of 501 (35.6%) and 96 out of 501 (12.3%)] (P¼0.0109 and P¼0.0268, respectively). The rates of cumulative usable and total expanded blastocysts at Day-5 plus Day-6 of development was statistically higher in Group 1 compared to the Group 2 (p¼0.0081and p¼0.0191, respectively).CONCLUSIONS: The results the current study demonstrate for the first time that the adjustment of the ORP levels in the embryo culture medium to physiological ORP levels through antioxidant supplementation is beneficial for older patients undergoing IVF treatments as it provides significantly increased number of usable and expanded blastocysts rates compared to the conventional embryo culture system.IMPACT STATEMENT: The adjustment of supraphysiological ORP levels normally found in commercial culture media to a physiological state may facilitate significantly improved rates of usable and expanded blastocysts during the IVF treatment of women advanced maternal age, which may increase their chances of getting pregnant.