Objectives: Using dental Ti implants has become a well-accepted and used method for replacing missing dentition. It has become evident that in many cases peri-implant inflammation develops. The objective was to create and evaluate the antibacterial effect of silver nanoparticle (Ag-NP) coated Ti surfaces that can help to prevent such processes if applied on the surface of dental implants. Methods: Annealing I, Ag ion implantation by the beam of an Electron Cyclotron Resonance Ion Source (ECRIS), Ag Physical Vapor Deposition (PVD), Annealing II procedures were used, respectively, to create a safely anchored Ag-NP layer on 1x1 cm 2 Grade 2 titanium samples. The antibacterial effect was evaluated by culturing Staphylococcus aureus (ATCC 29213) on the surfaces of the samples for 8 hours, and comparing the results to that of glass as control and of pure titanium samples. Alamar Blue assay was carried out to check cytotoxicity. Results: It was proved that silver nanoparticles were present on the treated surfaces. The average diameter of the particles was 58 nm, with a 25 nm deviation and Gaussian distribution, the the filling factor was 25%. Antibacterial evaluation revealed that the nanoparticle covered samples had an antibacterial effect of 64.6% that was statistically significant. Tests also proved that the nanoparticles are safely anchored to the titanium surface and are not cytotoxic. Conclusion: Creating a silver nanoparticle layer can be an option to add antibacterial features to the implant surface and to help in the prevention of peri-implant inflammatory processes. Recent studies demonstrated that silver nanoparticles can induce pathology in mammal cells, thus safe fixation of the particles is essential to prevent them from getting into the circulation.

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Though the antibacterial effect is advantageous, silver and silver nanoparticles can negatively affect the viability of human tissues. This study aims to check the viability of cells on surfaces with different particle size and to find the biologically optimal configuration. We investigated the effect of modified thickness of vaporized silver and applied heat and time on the physical characteristics of silver nanoparticle covered titanium surfaces. Samples were examined by scanning electron microscopy, mass spectrometry, and drop shape analyzer. To investigate how different physical surface characteristics influence cell viability, Alamar Blue assay for dental pulp stem cells was carried out. We found that different surface characteristics can be achieved by modifying procedures when creating silver nanoparticle covered titanium. The size of the nanoparticles varied between 60 to 368 nm, and hydrophilicity varied between 63 and 105 degrees of contact angle. Investigations also demonstrated that different physical characteristics are related to a different level of viability. Surfaces covered with 60 nm particle sizes proved to be the most hydrophilic, and the viability of the cells was comparable to the viability measured on the untreated control surface. Physical and biological characteristics of silver nanoparticle covered titanium, including cell viability, have an acceptable level to be used for antibacterial effects to prevent periimplantitis around implants.

A parodontális kezelések során a mechanikus terápia kiegészítésére alkalmazhatók olyan kémiai anyagok, melyekkela szájban fellelhető patogén baktériumok szaporodása visszaszorítható, a gyógyulási folyamat elősegíthető. Ennek egyikeszköze lehet a PerioChip® is, mint egy klórhexidin-glükonát (CHX) leadására képes rendszer. Jelen munkánk céljaezen rendszer hatóanyag-leadó tulajdonságának vizsgálata különböző pH-jú puffer oldatok alkalmazása során. Munkánkat4–12 pH tartományban végeztük el univerzális Britton-Robinson puffert, illetve 7,4-es pH-jú PBS puffer oldatotalkalmazva kioldódási közegként. A kioldódást 1 hét időintervallumon követtük figyelemmel, és a kioldódott CHX men�-nyiségét HPLC módszer segítségével határoztuk meg. A savas pH kedvező hatással volt a kioldódásra, gyors ütembenszabadult fel a hatóanyag 86,9%-a, de még pH 6 esetében is közel 80%-os volt a hatóanyag-leadás. Mindezek bázikuspH-n jelentősen kisebb értékeket mutattak, pH 8 esetében közel 40%, míg pH 10, és pH 12-nél már csak 30%-os nagyságrendbeestek. Eredményeink szerint a pH-változásnak jelentős hatása van a kioldódott CHX mennyiségére, és a kioldódásdinamikájára is.