Author contributions J.U., MT.B., A. LL. and A.B. designed and performed experiments and analysed data. M.Cl., M.Ca., E.F., C.F-P and M.G contributed to experiments. A.B-LL. analysed the public transcriptomic datasets of human samples and stats. E.B. and A.R.N. participated in data analyses. R.R.G conceived the project, designed and analysed data and supervised the overall project.

Purpose This study aims to identify the impact of affective components on behavioral intentions applying electronic word of mouth (eWOM) and is based on the “cognitive–affective–conative” model. EWOM allows researchers to get new insights about consumers’ behavior and explores new patterns of consumers’ decision-making processes. Design/methodology/approach This study is based on the theory of planned behavior (Ajzen, 1991), doubled with “cognitive–affective–conative” model (Oliver, 2014). This study applies structural topic modeling to examine the impact of satisfaction dimensions from all the Tripadvisor reviews on consumer behavioral intentions. The research sample covers all restaurants located in St. Petersburg (n = 10,424) and all consumers’ reviews (n = 286,642). Findings In this study, the dimensions of the affective component were identified. The results demonstrate that dimensions of the affective component (food quality, service quality, atmosphere and cost fairness) affect behavioral intention (willingness to share positive emotions). In total, 20 topics, forming these dimensions, have been indicated. Consumers tend to pay more attention toward food quality and restaurant staffs’ work when they are willing to share positive emotions and tend to point out auxiliary service when they have less willingness to share positive emotions. Random restaurant visits tend to increase the willingness to share positive emotions. Originality/value Research originality lies in a new methodological approach which is based on text mining techniques. To the best of the authors’ knowledge, this study is the first attempt to examine consumer behavior through the lens of the “cognitive–affective–conative” model based on eWOM and covers all businesses in the specific economic sphere. This has allowed the researchers to reveal new dimensions of consumer behavior and brought more insights into the consumers’ decision-making process.

<p>SF1: The expression of ANGPT2 and CXCR4 in CRC; SF2: Expression levels of ANGPT2, CXCR4, CXCL12, TEK and VEGFA in SW620_P and SW620_LiM2 cells; SF3: Expression levels of ANGPT2 and CXCR4 upon downregulation and upregulation.; SF4: CXCL1 and IL10 expression is controlled by RAS-ERK signaling pathway; SF5: Correlation of various genes expression and BRAF-signature or CRIS subtypes across CRC tumors; ST1: mRNA expression comparisons; ST2: Hypergeometric test for Signature FC-1.75.up ( 15 genes) - KEGG.</p>

<p>SF1: The expression of ANGPT2 and CXCR4 in CRC; SF2: Expression levels of ANGPT2, CXCR4, CXCL12, TEK and VEGFA in SW620_P and SW620_LiM2 cells; SF3: Expression levels of ANGPT2 and CXCR4 upon downregulation and upregulation.; SF4: CXCL1 and IL10 expression is controlled by RAS-ERK signaling pathway; SF5: Correlation of various genes expression and BRAF-signature or CRIS subtypes across CRC tumors; ST1: mRNA expression comparisons; ST2: Hypergeometric test for Signature FC-1.75.up ( 15 genes) - KEGG.</p>

<div>Abstract<p>Carcinoma development in colorectal cancer is driven by genetic alterations in numerous signaling pathways. Alterations in the RAS-ERK1/2 pathway are associated with the shortest overall survival for patients after diagnosis of colorectal cancer metastatic disease, yet how RAS–ERK signaling regulates colorectal cancer metastasis remains unknown. In this study, we used an unbiased screening approach based on selection of highly liver metastatic colorectal cancer cells <i>in vivo</i> to determine genes associated with metastasis. From this, an ERK1/2-controlled metastatic gene set (EMGS) was defined. EMGS was associated with increased recurrence and reduced survival in patients with colorectal cancer tumors. Higher levels of EMGS expression were detected in the colorectal cancer subsets consensus molecular subtype (CMS)1 and CMS4. <i>ANGPT2</i> and <i>CXCR4</i>, two genes within the EMGS, were subjected to gain-of-function and loss-of-function studies in several colorectal cancer cell lines and then tested in clinical samples. The RAS–ERK1/2 axis controlled expression of the cytokine ANGPT2 and the cytokine receptor CXCR4 in colorectal cancer cells, which facilitated development of liver but not lung metastases, suggesting that ANGPT2 and CXCR4 are important for metastatic outgrowth in the liver. CXCR4 controlled the expression of cytokines IL10 and CXCL1, providing evidence for a causal role of IL10 in supporting liver colonization. In summary, these studies demonstrate that amplification of ERK1/2 signaling in <i>KRAS</i>-mutated colorectal cancer cells affects the cytokine milieu of the tumors, possibly affecting tumor–stroma interactions and favoring liver metastasis formation.</p>Significance:<p>These findings identify amplified ERK1/2 signaling in <i>KRAS</i>-mutated colorectal cancer cells as a driver of tumor–stroma interactions that favor formation of metastases in the liver.</p></div>