No abstract

Microalgae are photosynthetic microorganisms that are widely distributed in waters. They have the potential as an alternative source of pigments, lipids, proteins, and carbohydrates. In this study, identification and isolation of single-cell microalgae from Ambon Bay were carried out by serial dilution method and inoculation of the culture by appearance/spread on the surface of the solidified agar medium. The results showed that the waters of Ambon Bay have physical parameters such as temperature, pH, salinity, and TDS, which are still within the threshold specified in the seawater quality standard for biota. The water conditions at the 10 sampling locations had different salinity, pH, temperature, and TDS. This condition affects the distribution of microalgae in the waters in Ambon Bay. The image from light microscopy shows that there are 27 species of microalgae scattered in the waters of Ambon Bay, which belong to the classes Chlorophyceae, Flagilariophyceae, Bacillariophyceae (diatoms), Coscinodiscophyceae, and Dinophyceae. Microalgae isolation obtained five species (single-cell) including four species from the class Bacillariophyta (Navicula sp, Nitzschia sp, Cyclotella sp) and one species from the class Chlorophyta (Chlorella sp).

Microalgae are photosynthetic microorganisms that contain carbohydrates can be converted into glucose through a hydrolysis process so that they can be used as raw materials for bioethanol production. Navicula sp. strain TAD is a type of marine microalgae that is spread in the inner Ambon Bay (TAD) sea waters and is used to manufacture bioethanol. This study aims to determine the content of carbohydrates and bioethanol produced from tropical marine microalgae Ambon bay Navicula sp. strain TAD. The research was conducted through several stages, namely the cultivation of Navicula sp. strain TAD to obtain biomass, determine carbohydrate content in biomass, hydrolyze biomass and manufacture bioethanol through a fermentation process using Saccharomyces cerevisiae. The results showed that Navicula sp. strain TAD cultivation for seven days had the highest cell density of 72.167 × 105 ± 0.946 cells  mL-1 with dry biomass of 0.933 ± 0.062 g and productivity of 0.166 ± 0.011 gL-1 hour-1. The sulfuric acid hydrolysis process was analyzed for a sugar content of 146.5695 ± 0.758 mg. A fermentation process follows the results of the hydrolysis to produce bioethanol. The bioethanol content obtained was 6.357%. These indicated that Navicula sp. strain TAD has the potential to produce bioethanol

Potensi kelautan di Maluku merupakan sarana penyediaan bahan makanan yang melimpah bagi masyarakat pesisir. Kelimpahan alga menjadi sumber pangan bagi masyarakat pesisir seperti sayur meti yang dimanfaatkan masyarakat pesisir pada Desa Liliboi, Maluku. Informasi tentang golongan jenis alga dan komposisi senyawa bioaktif dari sayur meti belum diketahui. Penelitian ini bertujuan untuk mengidentifikasi morfologi dan menguji kandungan fitokimia senyawa bioaktif pada sayur meti dari perairan pantai Desa Liliboi. Penelitian dilakukan melalui beberapa tahapan yaitu pengamatan morfologi, ekstraksi senyawa bioaktif dengan metode maserasi menggunakan pelarut metanol dan pengujian fitokimia senyawa bioaktif pada sayur meti dari perairan pantai Desa Liliboi. Hasil penelitian menunjukkan bahwa morfologi sayur meti ini memiliki bentuk sel yang mirip dengan alga dari devisi Chlorophyta genus Ulothrix. Ulotrix merupakan alga yang berbentuk filament. Sayur meti memiliki kandungan protein dan lipid masing-masing sebesar 31,782 0,057 mg/L dan 55,045 0,046%. Uji fitokimia menunjukkan bahwa ekstrak metanol mengandung senyawa bioaktif seperti alkaloid, flavonoid, steroid, saponin, dan fenol. Kandungan flavonoid ekstrak metanol relatif tinggi, yaitu 506,140±0,574 mg/L. Oleh karena itu, sayuran meti merupakan bahan kuliner yang sangat baik dengan potensi untuk digunakan sebagai bahan baku kosmetik, obat-obatan, dan bahan bakar nabati..