No abstract

The seeds of Nelumbo nucifera (Lotus) have been used in the traditional system of medicine for various ailments including diabetes. The present study was aimed at analyzing the levels of biologically important trace elements in the lotus seeds by atomic absorption spectroscopy and evaluating the hypoglycemic properties of seed ash on streptozotocin-induced diabetes in rats. Diabetic rats treated with lotus seed ash at a concentration of 200 mg/kg body weight orally for 30 days exhibited significant hypoglycemic activity. The presence of trace elements in appreciable amounts in the seeds may play a direct or indirect role on insulin secretion or its action in a synergetic manner. The hypoglycemic activity of the ash was comparable with glyclazide. The role of trace elements in disorders related to diabetes is also discussed briefly.

Objective: Due to the multifactorial and multisystemic nature of diabetes mellitus (DM), it is often treated with a combination of therapeutic agents. Earlier, we have synthesized and characterized several organozinc complexes and evaluated their safety and antidiabetic properties in experimental DM. In the present study, a new zinc mixed ligand (metformin-3-hydroxyflavone) was synthesized and characterized by various spectral studies and its antidiabetic properties were evaluated in high-fat diet (HFD) fed -low-dose streptozotocin (STZ)-induced Type 2 D (T2D) in rats. Methods:The zinc mixed ligand complex was characterized by spectral studies. The toxicity and dosage fixation studies were carried out as per OECD guidelines 423. HFD fed low-dose STZ-induced T2DM in rats was used as the experimental model. The hypoglycemic efficacy of the complex was evaluated through oral glucose tolerance test, homeostasis model assessment of insulin resistance (IR), QUICK I and by determining the status of important biochemical parameters. The activities of marker enzymes such as aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase were assayed. Metformin was used as a standard drug. Results:The spectral data evidenced the synthesis of a new zinc mixed ligand complex. The biochemical studies evidenced that the oral administration of the complex at a concentration of 10 mg/kg b.w/rat/day for 30 days to diabetic rats significantly improved the glucose homeostasis which was comparable to metformin treatment (50 mg/kg b.w). Conclusion:The zinc mixed complex possesses significant antidiabetic properties in ameliorating IR and stimulatory properties.

Due to the multifactorial and multisystemic nature of diabetes mellitus, it is often treated with a combination of therapeutic agents having different mode of action. Earlier, we have synthesized several organozinc complexes and evaluated their safety and antidiabetic properties in experimental type 2 diabetes mellitus (T2DM). More recently, we have synthesized a metformin-3-hydroxyflavone complex and studied its antidiabetic efficacy in experimental rats. In the present study, a new zinc-mixed ligand (metformin-3-hydroxyflavone) was synthesized, characterized by spectral studies and its antidiabetic properties was evaluated in HFD fed—low dose streptozotocin induced T2DM in rats. The hypoglycemic efficacy of the complex was evaluated through oral glucose tolerance test, homeostasis model assessment of insulin resistance, quantitative insulin sensitivity check index and by determining the status of important biochemical parameters. Oral administration of the complex at a concentration of 10 mg/kg body weight/rat/day for 30 days significantly improved the glucose homeostasis. The complex possesses significant antidiabetic properties relatively at a less concentration than metformin-3-hydroxyflavone complex in ameliorating hyperglycemia.

A novel class of Mn(III) and Fe(III) complexes of L-tyrosine-based ligand has been synthesized and characterized through various analytical and spectroscopic techniques. These complexes were found to exhibit efficient binding properties with the biomolecules viz. calf thymus DNA and BSA. The ability of complexes to bind with such biomolecules has been explored through absorption, emission and viscosity measurements. Based on spectroscopic techniques we can conclude that the complexes could bind to DNA via intercalation. It was observed that these complexes can cleave pBR322 DNA in gel-electrophoresis technique through oxidative mechanism. The BSA was quenched by the complexes around 340 nm adopting a mechanism of static mode. The binding constants, thermodynamic parameters and the donor to acceptor distance were calculated. Besides, molecular docking simulations were carried out for the complexes with human DNA topoisomerase and BSA protein. The docked poses are visualized to provide supportive evidence to the interaction of the synthesized complexes with DNA/BSA.