In this work, the experimental investigation on the performance and exergy analysis of mobile air conditioning system with suction line heat exchanger using environmental friendly HFO-1234yf was carried out under varied evaporator air flow rates. The performance was compared with existing HFC-134a results. The performance analysis showed that the cooling capacity and the coefficient of performance of the system with HFO-1234yf were lower than that of the HFC-134a by upto 2–11%. The power consumption and the volumetric efficiency of the compressor with HFO-1234yf were found to be 14.02% and 11.2% higher than that of HFC-134a. From the exergy analysis, it was observed that the major exergy destruction occurred in the compressor, followed by the condenser, evaporator, thermostatic expansion valve, and suction line heat exchanger for both refrigerants. The exergy efficiency of the system with HFO-1234yf was 2.4–12.6% lower than that of HFC-134a. From this study, it was observed that the losses experienced in the compressor, thermostatic expansion valve and evaporator lead to poor performance with HFO-1234yf.