The study's main objective is to limit bacterial biofilm formation on fixed orthodontic appliances. Bacterial biofilm formation on such devices (e.g., brackets) causes enamel demineralization, referred to as white spot lesions (WSL). WSL is significant health, social and economic problem. We provide a nanotechnology‐based solution utilizing a nanocomposite of gold nanoparticles embedded in a polyoxoborate matrix (BOA: B—boron, O—oxygen, A—gold, Latin aurum). The nanocomposite is fully inorganic, and the coating protocol is straightforward, effective, and ecologically friendly (low waste and water‐based). Prepared coatings are mechanically stable against brushing with a toothbrush (up to 100 min of brushing). Bacteria adhesion and antibacterial properties are tested against Streptococcus mutans—common bacteria in the oral cavity. BOA reduces the adhesion of bacteria by around 78%, that is, from around 7.99 × 105 ± 1.33 × 105 CFU per bracket to 1.69 × 105 ± 3.07 × 104 CFU per bracket of S. mutans detached from unmodified and modified brackets, respectively. Modified fixed orthodontic brackets remain safe for eukaryotic cells and meet ISO 10993‐5:2009 requirements for medical devices. The gathered data show that BOA deposited on orthodontic appliances provides a viable preventive measure against bacteria colonization, which presents frequent and significant complications of orthodontic treatment.
Background
The hazelnut (Corylus avellana) is still one of the most profitable nut crop species. In recent years, however, there has been growing interest in this species in the form of “fresh nuts” that are picked before falling out of the fruit cover. The aim of this study was to evaluate the effect of storage conditions for hazelnuts protected with biological preparations on selected morphological features of the fruits, their health status and the count of bacteria and fungi colonizing the fruits.
Results
The hazelnuts harvested from the trees protected with a preparation containing Pythium oligandrum and stored for 2 months under the controlled atmosphere conditions and in Xtend® bags (MAP) had the greatest weight and the highest percentage of the kernel. After 3 months of storage, the hazelnuts had reduced commercial value. Only a few hazelnuts displayed symptoms of infectious diseases caused by species of Botrytis and Monilia. The protection applied before the hazelnut harvesting contributed to a multiple increase in the bacterial and yeasts count on the husks and shells of the hazelnuts stored for 3 months. The bacterial count on the nuts stored under the controlled atmosphere (CA, 3%O2:3%CO2, a temperature of 0–1 °C, humidity of 85–95%) and under the controlled atmosphere conditions and in Xtend® bags (MAP) increased significantly. An analysis of the ITS region sequence revealed the presence of bacteria Arthrobacter luteolus and Pantoea agglomerans. A Koch test proved that both non-pathogenic bacteria and pathogenic fungi can cause the browning of the C. avellana leaf under conditions of high humidity. The application of a controlled atmosphere is recommended for a short-term storage of hazelnuts in the husk.
Conclusion
This research showed that 2 months’ storage of hazelnuts under controlled atmosphere conditions and Xtend® bags (MAP) prevented a reduction in the weight of hazelnuts in the husk, without the husk, and of the kernel and prevented the nut separation from the husk. In general, the application of biopreparations for the protection of the hazelnut had a positive effect on the kernel weight and size.
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