Chick embryo tracheal organ cultures showed increased resistance to infection by a coronavirus after exposure to ascorbate, while chick respiratory epithelium and allantois-on-shell preparations showed no increase in resistance to infection by an influenza virus or a paramyxovirus.
During attempts to establish tissue cultures from hepatopancreas, heart, and hemolymph of the giant tiger prawn (Penaeus monodon), using a medium including penicillin, streptomycin, and amphotericin B, bacterial contamination in the form of a sheet of growth attached to the tissue culture vessel was a persistent problem. Contaminant bacteria were teardrop-shaped cells arranged in rosettes, and electron microscopy revealed buds, crateriform structures, and the absence of a peptidoglycan layer in the cell wall, features characteristic of bacteria in the Planctomyces-Pirellula group, a phylogenetically distinct group of eubacteria. Two strains of contaminant bacteria were isolated in pure culture. Both exhibited morphology and antibiotic resistance consistent with their membership in the Planctomyces-Pirenlula group (order Planctomycetales) of eubacterig. Tissue culture media for marine invertebrates may select for such bacteria if high concentrations of cell wall synthesis-inhibiting antibiotics are included.
The objective of this paper is to analyse reduction in wind power variability through aggregation and use of energy storage systems. A key focus is to evaluate the impact of regulatory framework in addition to the capital expenditure to ascertain techno-economic feasibility of energy storage systems in wind farm applications. A generic techno-economic is developed which takes into account the effects of regulatory framework in addition to the technical and economic features of storage options. Existing wind farms from South Australia are used as test cases. First, a detailed quantitative analysis is performed to establish the variability associated with individual wind farms and the aggregations of their power outputs. Then, the appropriateness of a number of existing energy storage types are evaluated using the developed techno-economic model. Relationships between wind farm sizes, wind farm variability levels, storage capacity requirements, storage costs and storage payback times are determined and discussed for both current and potential future economic and regulatory scenarios. It is found that regulatory framework can be of paramount importance in ascertaining the economic feasibility of energy storage. For example, if the ramp-rate violation penalty (determined to be $8.89/MW/min) is doubled, then the payback time of energy storage capital investment is found to reduce from 5.32 years to 2.52 years. It is also found that larger wind farms require smaller energy storage capacity and smaller wind farms generally results in a shorter energy storage system payback times.
A baculovirus of Penaeus plebejus for which we propose the name Plebejus Baculovirus (PBV) was found in postlarval and juvenile P. plebejus from a hatchery and grow-out pond in New South Wales, Australia. Subsphencal eosinophilic inclusion bodies, mostly single or in pairs, were in hypertrophed nuclei of the hepatopancreas and mid-gut epithelia1 cells. Electron microscopy revealed a crystalline lattice of periobcity 20 nm in the inclusion bodies. Vinons up to 440 nm long, both free and occluded in the inclusion body, were scattered in the nuclex. The capsid envelope contained 2 electrondense layers.
Baculovirus particles were found in the digestive gland of Penaeus monodon from hatcheries and grow-out ponds in northern New South Wales, northern Queensland and the Northern Territory. Similar particles were seen in a Penaeus merguiensis specimen caught off Townsville, Queensland. The particles were found in the hypertrophied nuclei of hepatopancreatic epithelial cells, both free in the nucleoplasm and occluded in large eosinophilic intranuclear inclusion bodies. The nucleocapsids of the particles measured 45-52 nm × 260-300 nm and resembled the baculovirus reported from Penaeus plebejus.
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