ABSTRACT. Penaeus monodon (22.064 * 2.263 g) were exposed individually at 10, 20 and 30% salinity to 0 (control), 1, 5, 10 and 20 mg 1-' ammonia-N for 24 h. Changes in hemocyanin, protein and free amino acid levels, and whole shrimp ammonia-N excretion, urea-N excretion and dissolved organic N (DON) excretion were determined. Ammonia-N excretion decreased with increased salinity and with increased ambient ammonia-N. Ammonia-N accounted for 56.87. 70.43 and 78.13 % of total nitrogen excreted by P. monodon in 30, 20 and 10% respectively. DON increased to 67.26, 78.79 and 86.1% of total nitrogen excreted by the shrimp exposed to 20 mg 1-' ammonia-N in 30, 20 and 10°A, respectively. Net ammonia-N uptake occurred as shrimp were exposed to ambient ammonia-N greater than 5 mg 1-I at all salinity levels tested. Hemocyanin and protein levels decreased, whereas hemolyrnph ammonia, urea, total free amino acid levels and taurine level increased, with increased ambient ammonia-N. Following exposure to ambient ammonia-N, P. rnonodon changed its excretory pattern, accumulation of hemolymph ammonia, and catabolism of hemocyanin and protein to balance osmoregulation.

Stahlhut and Hymowitz (1983) found five inhibitors-I, II, III, IV, and V-from cultivar Amsoy 71. Earlier we (Tan-Wilson et al., 1985a) studied the distribution of Bowman-Birk class inhibitors in eight soybean strains and found isoinhibitors that we designated as BBSTI-A, -A', -A", -B, -B', -C, -C', -D, -E, and -E', in order of increasing mobility upon polyacrylamide gel electrophoresis in the Davis system.