Contents:This report presents results of several experiments done with Angora, Alpine and Saanen goats in order to define the actual feasibility of production, storage and transfer of embryos in this species. The superovulatory regimen used pFSH administered a t the end of a progestagen treatment (45 mg FGA vaginal sponges, 11 days). When 16 mg Armour equivalent are administered t o dairy goats the numbers of CL and of transferable embryos are significantly higher (P < 0.05) if the FSHILH ratio decreases from first to last injection (8-1 -0.4) compared to constant FSHILH ratio ( I ) , (CL: 13.0 us 6.8, transferable embryos: 4.9 us 1.6). During commercial activity the production of transferable embryos was found to be lower (after 16 mgpFSH and surgical recovery) in Angora compared t o Aipine goats (5.2 vs 6.7) and (after 20 mgpFSH and laparoscopic recovery) in Angora goats treated during 3 days compared to 4 days (4.2 us 5.7). The level of stimulation induced by 16 mg pFSH with decreasing FSHILH ratio in dairy goats does not differ significantly between breeding and out of breeding season (CL: 13.7 us 11.9, transferable embryos: 4.7 us 4.0). Repeating this treatment every 7 weeks decreases the response (1st treatment: 12.8 CL, 4.3 transferable embryos; 2nd t o 5 t h treatment: 8.6 CL, 1.9 transferable embryos, P < 0.05). After 19.9% of the treatments large unovulated follicles were observed on the ovaries a t the time of embryo recovery and for 10% some or all of the CL were regressing (Corpora albicans). Oestrus was observed 24 to 5 4 h after sponge removal and this interval affected ovulation rates (24 h: 14.4 CL; 3 0 h: 9.6 CL; 4 8 h 8.6 CL; P < 0.01). 71 and 60.2% of the eggs are fertilized after hand mating or A I respectively. Timing of A I and number of CL influenced the fertilization rate (65.2% f o r A I at the beginning of oestrus us 52.1 % for A I at the end of oestrus, P < 0.05; 66.1% f o r < 15 CL us 48.8% f o r > 15 CL, P < 0.01). The use of laparoscopy has been developed f o r embryo recovery and transfer. The recovery rate is lower (62 us 85%) but each donor can be collected more than 7 times without severe adhesions. After transfer pregnancy rate is higher after surgery (74.4% us 42.5% P < 0.01). Inhalt: Gewinnung, Triefgefrieren und Transfer von Embryonen bei Angora-, Alpenund Saanenziegen A n den genannten Ziegenrassen wurden in verschiedenen Experimenten neuere Methoden zur Gewinnung, Konservierung und Transfer von Embryonen erarbeitet. Zur Superovulationsauslosung wurde pFSH am Ende einer Progestagenbehandlung (45 mg U.S.
Contents In order to analyze the effects of repeated use of progestagen‐PMSG treatment, estrus and pregnancy results have been analyzed for 1989 in a Saânen dairy goat herd in which breeding takes place each year out of season after FGA/PMSG treatment. After the first 1989 treatment (169 goats), percentage of goats showing estrus and kidding have been lower for 59 multiparous than for 46 primiparous and 64 nulliparous females. Moreover, when 38 goats are treated for a second time in 1989, 44.7% exhibited estrus vs 71.0% after the first treatment (P < 0.05). The PMSG binding level before the 1st 1989 treatment is higher for multiparous (17.5 ± 23.1%) than nulli and primiparous (‐0.06 ± 0.7 and 1.2 ± 1.9%) and is increased for all parities after treatment (23.2 ± 26.4 after vs 5.7 ± 15.0% before, P < 0.01). For nulliparous and primiparous females; PMSG binding levels are not different for pregnant or not pregnant nulliparous and primiparous goats. On the opposite, PMSG binding rates are higher in non pregnant (25.7 ± 23.3) than in pregnant multiparous goats (6.5 ± 15.9) (P < 0.01). However, when the binding rate is ≤ 5.12% (computerized distributions) multiparous goats exhibit estrus and pregnancy at levels not different from nulliparous or primiparous females (% estrus 95.8 vs 100 or 97.8%, % pregnancy 66.7 vs 70.3 and 63.0% respectively). Repeated use of PMSG during the female life or during one given year leads to active immunization against PMSG (as measured by percentage of binding of PMSG in plasma) decreasing the efficiency of ovarian stimulation out of breeding season. Inhalt: Einfluß einer wiederholten Progestagen/PMSG Behandlung zur Östruskontrolle außerhalb der Paarungssaison bei Milchziegen Um den Einfluß wiederholter Progestagen‐PMSG‐Behandlung zu prüfen, wurden Östrus und Trächtigkeitsergebnisse in einer Saanen Ziegenherde für das Jahr 1989 analysiert. Bei den untersuchten Tier en wird die Bedeckung jedes Jahr außerhalb der Saison nach FGA/PMSG‐Behandlung vorgenommen. Nach der ersten Behandlung 1989 (169 Ziegen) war der Prozentsatz Ziegen, die einen Östrus zeigten und ablammten, bei 59 multiparen niedriger als bei 46 primiparen und 64 nulliparen Tieren. Darüber hinaus zeigten von 38 Ziegen nach einer zweiten Behandlung 1989 zu 44,7% einen Östrus gegenüber 71% nach der ersten Behandlung (p < 0,05). Die Bindungsfähigkeit für PMSG war bei der ersten Behandlung bei den multiparen Tieren höher (17,5 ± 23,1%) als bei nulli‐ und primiparen Tieren (‐0,06 ± 0,7% und 1,2 ± 1,9%) und stieg für alle Tiere nach der Behandlung an (23,2 ± 26,4% nach zu 5,7 ± 15,0% vor der Behandlung, p < 0,01). Die PMSG Bindungsfähigkeit war nicht unterschiedlich bei tragenden und nichttragenden nulli‐ und primiparen Tieren. Dagegen waren die PMSG Bindungsraten bei nichttragenden Ziegen (25,7 ± 23,3%) höher als bei tragenden multiparen Tieren (6,5 ± 15,9%) (p < 0,01). Wenn allerdings die Bindungsraten unter 5,12% (Computerverteilung) sinkt, zeigen multipare Ziegen Östrus und Trächtigkeitsraten, die sich von multiparen und primipar...
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