Laparoscopic ovum pick-up and in vitro production of sika deer embryos: Effect of season and culture conditions

Locatelli, Yann; Vallet, J.C.; Huyghe, F.; Cognie, Y.; Legendre, X.; Mermillod, Pascal

doi:10.1016/j.theriogenology.2006.05.005

Cited by 27 publications

(22 citation statements)

References 31 publications

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“…In that sense, LOPU rapidly became, and remains as, the method of choice for the collection of immature oocytes for IVEP in sheep and goats (Baldassarre et al, 1994;Stangl et al, 1999;Baldassarre et al, 2002;Cognie et al, 2004). Moreover, LOPU-IVEP has also been applied for oocyte collection in wild life species including deer and big felines (Miller et al, 1990;Locatelli et al, 2006;Baldassarre et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Baldassarre

Bordignon

2018

Anim Reprod

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Laparoscopic ovum pick-up (LOPU) conducted on bovine and buffalo calves of 2-6-month of age, followed by in vitro embryo production and transfer into synchronous adult recipients, is a powerful tool for accelerated genetic gain and early dissemination of top genetics. In its current state, the technology is characterized by higher oocyte recovery rates, lower oocyte-to-embryo yields, and similar pregnancy and term development rates compared with adult counterparts. Improvements in oocyte competence have been made in recent years mainly through gonadotropin stimulation protocols tailored for prepubertal donors. These advances have brought the technology to the point of been apt for commercial application. However, future research must focus on increasing the proportion of fully competent oocytes recovered from calves thereby further empowering the role this technology platform can play in programs for accelerated dissemination of superior genetics.

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Section: Introductionmentioning

confidence: 99%

Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Baldassarre

Bordignon

2018

Anim Reprod

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“…In Experiment 2, the proportion and quality of embryos produced in vitro were not different between the anovulatory and ovulatory seasons. Studies had previously shown a negative effect of the anovulatory season on in vitro embryo production in sheep (Stenbak et al, ) and sika deer (Locatelli et al, ). It may be that altered hormonal profiles during the non‐breeding season compromised oocyte quality in these species (e.g.…”

Section: Discussionmentioning

confidence: 97%

Effect of season and superstimulatory treatment on in vivo and in vitro embryo production in wood bison (Bison bison athabascae)

Palomino

Mastromonaco

Cervantes

et al. 2019

Reprod Domestic Animals

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Two experiments were done using a two-by-two design to determine the effects of season and superstimulatory protocol on embryo production in wood bison. In Experiment 1 (in vivo-derived embryos), ovarian superstimulation was induced in female bison during the ovulatory and anovulatory seasons with either two or three doses of FSH given every-other-day (FSH × 2 vs. FSH × 3, respectively). Bison were given hCG to induce ovulation, inseminated 12 and 24 hr after hCG, and embryos were collected 8 days after hCG (n = 10 bison/group). In Experiment 2 (in vitro embryo production), ovarian superstimulation was induced in female bison during the ovulatory and anovulatory seasons with two doses of FSH, and in vivo maturation of the cumulus-oocyte complexes (COC) was induced with hCG at either 48 or 72 hr after the last dose of FSH. COC were collected 34 hr after hCG, and expanded COC were used for in vitro fertilization and culture. In Experiment 1, the number of follicles ≥9 mm, the proportion of follicles that ovulated, the number of CL, and the total number of ova/embryos collected did not differ between seasons or treatment groups, but the number of transferable embryos was greater (p < .05) in the ovulatory season. In Experiment 2, no differences were detected between seasons or treatment groups for any end point. The number of transferable embryos produced per bison was greatest (p < .05) using in vitro fertilization and was unaffected by season (1.5 ± 0.2 and 1.1 ± 0.3 during anovulatory and ovulatory seasons, respectively), in contrast to in vivo embryo production which was affected by season (0.1 ± 0.01 and 0.7 ± 0.2 during anovulatory and ovulatory seasons, respectively). Results demonstrate that transferable embryos can be produced throughout the year in wood bison by both in vivo and in vitro techniques, but the efficiency of embryo production of in vivo-derived embryos is significantly lower during the anovulatory season. K E Y W O R D S FSH, in vitro embryo, in vivo embryo, season, wood bison | 55 PALOMINO et AL. How to cite this article: Palomino JM, Mastromonaco GF, Cervantes MP, Mapletoft RJ, Anzar M, Adams GP. Effect of season and superstimulatory treatment on in vivo and in vitro embryo production in wood bison (Bison bison athabascae).

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“…The main criterion of quality was the preservation of the proper morphology of the recovered ovular cells and their ability to develop and be raised in vitro. There are different criteria used for evaluation and classification of oocytes, from two to six degrees [29][30][31][32][33][34][35]. The best quality oocytes are those with homogenous cytoplasm, with corona radiata and a thick layer of cells of cumulus oophorus (COC-cumulus oocyte complexes).…”

Section: Discussionmentioning

confidence: 99%

“…Slightly worse quality oocytes may be qualified for IVM/IVF as well, including those with homogenous cytoplasm, with corona radiata and with an expanded layer of cells of cumulus oophorus (expanded COCs). There are some authors who qualify for IVM/IVF oocytes without granulosa cells (DCOCs, cumulus-denuded oocytes), with proper cytoplasm [29,30]. A similar evaluation and classification scale of oocytes was used by Koeman et al [31] and Locatelli et al [30].…”

Section: Discussionmentioning

confidence: 99%

L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method

Wieczorek

Koseniuk²,

Skrzyszowska

et al. 2020

Animals

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The laparoscopic method of recovering oocytes in goats and sheep is one of the minimally invasive methods used in the biotechnology of animal reproduction. It allows for good quality oocytes that are suitable for in vitro maturation and fertilization to be recovered. The limitation of using the laparoscopic ovum pick-up (L-OPU) method in goat and sheep is its changing effectiveness and the lack of repeatability of results, as well as the varying effectiveness of different variants of the method. Therefore, it is necessary to develop effective non-invasive techniques allowing for multiple good quality oocyte recovery that would be suitable for in vitro maturation and fertilization. In this study, four different L-OPU variants were described in goats and sheep. Various techniques of recovering oocytes were discussed, including the techniques of conducting the operation, various tools for recovering oocytes, and different plans of hormonal stimulation. Recovery rates were 35% (Variant I), 57% (Variant II), 72% (Variant III), and 67% (Variant IV). After evaluation, 94% (both Variant I and II), 93% (Variant III), and 84% (Variant IV) of the oocytes were qualified for in vitro maturation. The results of the study show that the proposed technique of laparoscopic recovery of oocytes allows a sufficient number of ovarian cells suitable for in vitro culture to be obtained and as a consequence it makes them useful in in vitro maturation/in vitro fertilization (IVM/IVF) programs or cloning. The method allows for a fast and effective conduct of the operation in a living donor with minimal invasiveness while preserving the excellent condition of animals.

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Laparoscopic ovum pick-up and in vitro production of sika deer embryos: Effect of season and culture conditions

Cited by 27 publications

References 31 publications

Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Effect of season and superstimulatory treatment on in vivo and in vitro embryo production in wood bison (Bison bison athabascae)

L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method

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