Over 2004Over -2006, effects of mineral fertilisers (60 N + 30 P + 60 K kg/ha or 120 N + 30 P + 60 K kg/ha) and farmyard manure (12 or 24 t/ha) application on biological and chemical properties of soil were studied in seminatural grassland. The research trial was established at Radvaň site, central Slovakia (altitude 480 m, loamy to sandy-loamy Cambisol). These parameters were investigated during the growing seasons: CO 2 production, intensity of total N mineralisation (TMN), the rate of nitrification (NIT), total C content in microbial biomass (MB-C), C ox , N t , P, K, Mg and the humic acids to fulvic acids ratio (HA:FA). At the application of mineral fertilisers, the highest N rate significantly increased TMN and NIT, mainly in 2004 and 2005. The manure application showed the strongest effects on the biological parameters. The manure rate of 12 t/ha significantly increased the soil respiration in 2005 and 2006 as well as TMN and NIT throughout the research. The manure application increased also the soil pH, MB-C, N t , Mg, P and C ox .

This study highlights the effect of drought and ambient temperature on performance and herbage quality of legume monocultures and grass-legume mixtures. In a field experiment, the total dry matter yield, seasonal pattern of dry matter yield distribution, content of crude protein and crude fibre of monocultures of red clover and alfalfa and grass-legume mixtures were investigated during two consecutive dry years (2011-2012). Alfalfa cultivars Kamila and Tereza grown as monocultures or as mixtures with Festulolium braunii (cultivar Achilles) outperformed the red clover cultivars Fresko and Veles and provided a well-balanced total and seasonal dry matter yield during both years. Across all experimental years, crude protein content was significantly higher at alfalfa monocultures and mixture when compared with clover monocultures (P < 0.05). However, considerable lower content of crude fibre at clover monocultures in comparison with alfalfa ones was found. Responses of nutritive parameters of both legume species to weather variables were different. Crude protein content in red clover was independent of rainfall and temperature. In contrast, the crude fibre content correlated with temperature whereby the alfalfa monocultures showed stronger correlations (P < 0.05) than red clover monocultures.

This paper aimed to monitory the dry matter biomass production and carbon stocks of above-and below-ground biomass in five types of grasslands in Slovakia: i) lowland oversowed pasture ii) lowland hay meadows, iii) mesophilous pasture, iv) mountain hay meadows, v) abandoned grassland. Averaged over two cropping seasons the total above-and below-ground biomass differed significantly across the monitored grasslands. It ranged respectively from 2.18 to 7.86 t/ha and from 9.64 to 22.67 t/ha dry matter depending on the pedoclimatic condition and the botanical composition of each grassland type. Consequently, this resulted also in the carbon stocks in above-and below-ground biomass. Generally, the mean carbon stocks were 1.56 t/ha for above-ground biomass (24%) and 4.83 t/ha for below-ground biomass (76%). The botanical composition for all the grassland types was also described. The highest number of plant species (55) was observed in lowland hay meadow located in Slovak Karst, the lowest one (23) for the oversowed grassland located in Eastern Slovak Upland. This monitoring paper showed that semi-natural grassland habitats and improved grasslands as well are an important carbon sink, and they can play a key role in global climate change mitigation.