Sixteen multiparous Holstein cows (weighing 600 +/- 50 kg, in 184.8 +/- 52 d of lactation, and at 101.9 +/- 43 d of gestation) were confined to wooden metabolic cages and exposed to a vertical electric field of 10 kV/ m and to a uniform horizontal magnetic field of 30 microT (microtesla). The trial was conducted as a switch-back statistical design. Cows were divided into two replicates of 8 cows each. One replicate was exposed for three periods of 28 d each. During the first period, the electric and magnetic fields were off; during the second period, they were on; and, during the final period, they were off. The second replicate was exposed for three periods also, but the activity of the fields was reversed (first period, on; second period, off; and third period, on). Blood samples were obtained twice weekly for the determination of cortisol and progesterone and once weekly for the determination of pH and blood gases. Milk samples were collected once weekly to determine milk components (fat, protein, SNF, and SCC). Milk yield and feed consumption were measured daily. Most of the variables studied (bicarbonate, pH, O2 and CO2 partial pressures, cortisol concentration in blood, uncorrected milk yield, and milk components other than milk fat) showed no variation that could be attributed to exposure to electric and magnetic fields. Associations were found between the electric and magnetic fields and increased DMI, 4% FCM yield, milk fat content, and plasma progesterone.

Sixteen multiparous, pregnant, lactating Holstein cows (weighing 600 +/- 50 kg, at 184.8 +/- 52 d of lactation, and at 101.9 +/- 43 d of gestation) were confined to wooden metabolism cages and exposed to a vertical electric field of 10 kV/m and a uniform horizontal magnetic field of 30 microT. The trial was conducted using a switchback statistical design. Cows were divided into two sequence groups of 8 cows each. One sequence group was exposed for three periods of 28 d each. The electric and magnetic fields were off during the first period, on during the second period, and off during the final period. The second sequence group was exposed for three periods also, but the activity of the fields was reversed (on during the first period, off during the second period, and on during the third period). On d 25 of each exposure period, blood samples were obtained every 0.5 h for 14 h starting at 1700 h to determine melatonin concentration. Nocturnal melatonin concentrations did not show any variation that could be attributed to exposure to electric and magnetic fields.

Two experiments were conducted to test the hypothesis that electric and magnetic field (EMF) exposure may result in endocrine responses similar to those observed in animals exposed to long days. In the first experiment, 16 lactating, pregnant Holstein cows were assigned to two replicates according to a crossover design with treatment switchback. All animals were confined to wooden metabolic cages and maintained under short day photoperiods (8 h light/16 h dark). Treated animals were exposed to a vertical electric field of 10 kV/m and a horizontal magnetic field of 30 microT (EMF) for 16 h/day for 4 weeks. In a second, similar experiment, 16 nonlactating, nonpregnant Holstein cows subjected to short days were exposed to EMF, using a similar protocol, for periods corresponding to the duration of one estrous cycle. In the first experiment, circulating MLT concentrations during the light period showed a small numerical decrease during EMF exposure (P < .05). Least-square means for the 8 h light period were 9.9 versus 12.4 pg/ml, SE = 1.3. Melatonin concentrations during the dark period were not affected by the treatment. A similar trend was observed in the second experiment, where MLT concentrations during the light period tended to be lower (8.8 pg/ml vs. 16.3 pg/ml, P < .06) in the EMF exposed group, and no effects were observed during the dark period. Plasma prolactin (PRL) was increased in the EMF exposed group (16.6 vs. 12.7 ng/ml, P < .02) in the first experiment. In the second experiment, the overall PRL concentrations found were lower, and the mean plasma PRL concentration was not affected by treatment. These experiments provide evidence that EMF exposure may modify the response of dairy cows to photoperiod.

Electric and magnetic fields (EMF) are generated by the transmission of electricity through high tension lines traversing rural areas. Previous studies showed increased dry matter intake (DMI) and fat corrected milk in dairy cows exposed to EMF. Because EMF exposure has been shown to suppress pineal release of melatonin in some species, it was hypothesized that EMF effects resemble those of exposure to long days. Previous studies have shown that DMI and milk production increase in dairy cattle in response to long day photoperiods, and this has been observed in association with increased circulating insulin-like growth factor 1 (IGF-1), but not growth hormone (GH). The hypothesis that EMF act by modifying the response to photoperiod was tested by subjecting dairy cows to controlled EMF exposure while keeping them under short-day conditions. Sixteen lactating, pregnant Holstein cows were exposed to a vertical electric field of 10 kV/m and a horizontal magnetic field of 30 microT in a crossover design with treatment switchback. Two groups of eight cows each were exposed to EMF for 16 h/d in either oftwo sequences. Each sequence consisted of three consecutive 28-d periods. All animals were maintained under short day conditions (8 h light, 16 h dark) during the trial. DMI and plasma IGF-1 were increased (P < 0.01) during EMF exposure (17.03 vs.16.04 kg/d, SE = 0.4; 137 +/- 6 ng/ml vs 126 +/- 6, respectively). The mean GH concentration was not affected, but a treatment x hour interaction was detected, with GH lower for the EMF exposed animals during the first 16 h of the sampling period, and higher for the last 8 h. Overall, the yield of milk or its components was not affected by EMF exposure, but milk yield was significantly higher for the exposed animals during wk 4 of treatment.

Graphical Abstract Summary: Cows are typically milked 2 or more times on a test-day, but not all those milkings are measured. Statistical methods have been proposed to estimate daily yields, centering on various yield correction factors in 2 broad categories: additive correction factors (ACF) and multiplicative correction factors (MCF). This research note presented a technical review of statistical models for estimating daily milk yields concerning their statistical interpretations, mode assumptions, and challenges. An exponential regression model was proposed as an alternative tool for estimating daily milk yields. The features of ACF and MCF are illustrated using simulation datasets, and their performance was evaluated by 10-fold cross-validations. The methods were described explicitly for estimating daily milk yield in morning (AM) and evening (PM) milking plans, but the principles are generally applicable to cows milked more than 2 times a day, and apply to estimating daily fat and protein yields, with some necessary modifications.