Introduction: Atypical Squamous Cells(ASC) is a common gynaecologic cytologic abnormality, comprising around 5% of Papanicolaou test results. It reflects a diagnosis of uncertainty and is used as an intra-laboratory & inter-laboratory comparison tool for quality control purposes. For this purpose, ASC/SIL ratio can be used as a quality control measure. Bethesda system suggests that ASC/SIL ratio for an individual or laboratory should be less than 2:1 or 3:1. Objectives:To assess the ratio of ASC/SIL categories and thereby evaluation of quality control in gynaecologic cytology smears. Methods:The present study being a retrospective study was conducted in the Dept. Results: Of the total 436 cases, 226 cases were ASCUS and ASC-H and 210 cases were LSIL, HSIL and SCC. ASC/SIL ratio was obtained by dividing the sum of all ASC cases by the sum of all SIL cases. The ratio obtained was 1.1:1 which is below the upper bench mark of 3:1. Conclusion:Monitoring the ASC/SIL ratio of a laboratory is a useful quality control measure. Bethesda system have suggested that the ratio should be less than 3 and others have suggested that lower ratios are desirable. As diagnosis of ASCUS conveys uncertainty, a low ratio decreases the uncertainty produced by laboratory and may reduce the percentage of women with negative biopsy results.