No abstract

It could be shown that using multivariate analysis of variance it is possible to find the optimal set of serum lipid parameters, containing serum esterified cholesterol, serum total cholesterol and high density lipoproteins esterified cholesterol, to differentiate healthy persons from patients affected by ovarian cancer.

Sera from normal persons and patients with acute viral hepatitis were mixed with indocyanine green (ICG), then fractionated on Sephadex G-200 columns. Alternate portions of the effluent were examined spectrophotometrically at 280 nm (for protein content) and at 800 nm (for ICG concentration). In most normal sera, three distinct ICG peaks were distinguished, the first and third corresponding exactly to the position of protein peaks, the second being most frequently shifted slightly toward heavier protein fractions. By graphic analysis, up to three additional masked peaks were usually detected in ICG curves. The individual fractions on ICG curves were numbered from I to VI, beginning with the fraction of greatest molecular weight. In the acute phase of viral hepatitis, the ICG curve was characteristically changed: fraction IV decreased significantly or even disappeared, and fraction I simultaneously increased. Fractions III and V also increased, but less. These changes in the ICG curves may result from interaction between serum proteins and liver cell proteins originating from damaged liver cells, an interaction leading to the formation of complexes of high molecular weight.

The method presented allows the separation and fractionation of up to 12 subfractions of human serum small molecular diameter lipoproteins (SMDL) by polyacrylamide gel electrophoresis. The method was checked and confirmed by more than 2000 separations. The frequency of the appearence of the particular subfractions was investigated in 300 persons-children and adults. The method of quantitative estimation of each subfraction was evaluated. The precision of this method was 3 % to 11 % (mean 6 %). A good reproducibility of separations was found within 2 to 4 days after blood collection (with refrigeration). The stability of the separation patterns was satisfactory for up to 9 days in five healthy persons. Examples of differences in the SMDL-subfraction patterns, depending on sex, age and pathology, are given. Eine einfache Methode zur quantitativen Bestimmung der Subfraktionen von Lipoproteinen geringen Molekuldurchmessers (SMDL) im Serum vom Menschen Zusammenfassung: Die vorgestellte Methode erlaubt die Trennung von Lipoproteinen geringen Moleküldurchmessers im Serum vom Menschen in bis zu zwölf Subfraktionen von unterschiedlicher elektrophoretischer Beweglichkeit in Polyacrylamidgel. Die Methode wurde an mehr als 2000 Trennungen geprüft und bestätigt. Die Häufigkeit des Auftretens einzelner Subfraktionen wurde bei 300 Probanden-Erwachsenen und Kindern-untersucht. Die Methode der quantitativen Bestimmung jeder Subfraktion wurde geprüft. Die Präzision der Methode betrug 3-11 % (x = 6 %). Eine gute Reproduzierbarkeit der Trennungen wurde innerhalb 2-4 Tagen nach Probennahme bei Probenverwahrung im Kühlschrank gefunden. Zufriedenstellende Stabilität der Trennbilder innerhalb neun Tagen wurde durch je fünf Untersuchungen von Gesunden bestätigt. Einige Beispiele für SMDL-Subfraktionen-BUder bei verschiedenem Alter, Geschlecht.und Erkrankungen werden gegeben.

The ADVIA (Bayer) haematological system differentiates leukocytes by their volume and their peroxidase (MPO and EPO) activity. It thus allows specific counting of the eosinophils and detection of totally EPO and MPO deficient individuals. EPO activity is determined by the coordinates of the eosinophil cluster on the outprint. A reliable method of quantitation by simple angle measure is suggested. It allows recognition of partial deficiencies. Among approximately 100,000 persons, 29 cases of total EPO deficiency were detected. No typical or specific pathology of the defect was noted. Family studies showed heterozygous parents and offspring to be partially deficient. The frequency of these partial deficiencies in the general population is studied. The results exclude a simple autosomal recessive monogenic transmission of the defect. A model for bigenic transmission is suggested.