In today’s information society, with the growing integration of the Internet into individuals’ lives, problematic Internet use (PIU) among adolescents has become more prevalent. Therefore, we conducted this study to investigate the correlation between active parental mediation and PIU in adolescents, as well as the potential mediating roles of parent–child relationship and adolescents’ hiding online behavior. A total of 539 middle school students (mean age = 13.384) were recruited for this study and participated by completing a series of paper-and-pencil questionnaires. The findings indicated a significant negative relationship between active parental mediation and PIU. Furthermore, both the mediating role of the parent–child relationship and the role of hiding online behavior were found to be significant. Specifically, the mediating role is comprised of two paths: the independent mediating role of the parent–child relationship, and the sequential mediating role involving both the parent–child relationship and hiding online behavior. The study contributes an innovative theoretical perspective to deepen the understanding of the formation mechanism of PIU. Moreover, it offers practical empirical insights for the prevention and intervention of PIU among adolescents.

We present the detailed study of the digital readout of Topmetal-II -CMOS pixel direct charge sensor. Topmetal-IIis an integrated sensor with an array of 72 × 72 pixels each capable of directly collecting external charge through exposed metal electrodes in the topmost metal layer. In addition to the time-shared multiplexing readout of the analog output from Charge Sensitive Amplifiers in each pixel, hits are also generated through comparators with individually DAC settable thresholds in each pixel. The hits are read out via a column-based priority logic structure, retaining both hit location and time information. The in-array column-based priority logic is fully combinational hence there is no clock distributed in the pixel array. Sequential logic and clock are placed on the peripheral of the array. We studied the detailed working behavior and performance of this readout, and demonstrated its potential in imaging applications.

High precision timing distribution is crucial to many large scale cosmology and particle physics experiments. Besides the space and energy information, the accurate timing provides an extra dimension for physics event reconstruction. In the timing distribution system, accurate clock phase measurement is an indispensable tool to monitor the phase drift and to achieve accurate phase adjustment. This paper introduces a novel phase measurement method implemented in the Xilinx Field Programmable Gate Array (FPGA). It uses the dedicated phase interpolator in the multi-gigabit transceiver. A design based on this method is implemented within the Kintex Ultrascale series FPGA. The preliminary test result shows that a sub-picosecond level precision is achieved. With this system, the nonlinearity of the phase adjustment in the Xilinx transceiver is measured.

Cytochrome P450nor catalyzes an unusual reaction that transfers electrons from NADP/NADPH to bound heme directly. To improve the expression level of P450nor2 from Cylindrocarpon tonkinense (C.P450nor2), Escherichia coli system was utilized to substitute the yeast system we constructed for expression of the P450nor2 gene, and the protein was purified in soluble form using Ni(+)-NTA affinity chromatography. In contrast to P450nor from Fusarium oxysporum (F.P450nor) and P450nor1 from Cylindrocarpon tonkinense (C.P450nor1), C.P450nor2 shows a dual specificity for using NADH or NADPH as electron donors. The present study developed a computational approach in order to illustrate the coenzyme specificity of C.P450nor2 for NADH and NADPH. This study involved homology modeling of C.P450nor2 and docking analyses of NADH and NADPH into the crystal structure of F.P450nor and the predictive model of C.P450nor2, respectively. The results suggested that C.P450nor2 and F.P450nor have different coenzyme specificity for NADH and NADPH; whilst the space around the B'-helix of the C.P450nor2, especially the Ser79 and Gly81, play a crucial role for the specificity of C.P450nor2. In the absence of the experimental structure of C.P450nor2, we hope that our model will be useful to provide rational explanation on coenzyme specificity of C.P450nor2.

Cytochrome P450nor catalyzes an unusual reaction that transfers electrons from NADP/NADPH to bound heme directly. To improve the expression level of P450nor2 from Cylindrocarpon tonkinense (C.P450nor2), Escherichia coli system was utilized to substitute the yeast system we constructed for expression of the P450nor2 gene, and the protein was purified in soluble form using Ni + -NTA affinity chromatography. In contrast to P450nor from Fusarium oxysporum (F.P450nor) and P450nor1 from Cylindrocarpon tonkinense (C.P450nor1), C.P450nor2 shows a dual specificity for using NADH or NADPH as electron donors. The present study developed a computational approach in order to illustrate the coenzyme specificity of C.P450nor2 for NADH and NADPH. This study involved homology modeling of C.P450nor2 and docking analyses of NADH and NADPH into the crystal structure of F.P450nor and the predictive model of C.P450nor2, respectively. The results suggested that C.P450nor2 and F.P450nor have different coenzyme specificity for NADH and NADPH; whilst the space around the B'-helix of the C.P450nor2, especially the Ser79 and Gly81, play a crucial role for the specificity of C.P450nor2. In the absence of the experimental structure of C.P450nor2, we hope that our model will be useful to provide rational explanation on coenzyme specificity of C.P450nor2.