Rainbow trout were sprint-trained (30 s duration) once or twice on alternate days for a period of 6 weeks. Swim speed for the first 10 s of a training bout averaged 11.4 bl s ' for group 2 (trained once) and 10.2 bl s -' for group 3 (trained twice). Food consumption, growth rate and conversion efficiency were measured over 2-week periods. Food consumption was 31-38% less for the trained groups than for the control group (group I). The growth rates of control and trained fish increased gradually over the training period. The growth rate of trained fish was always significantly less (48-81%) than that of control fish. Although conversion efficiency was significantly less for group 3 at the beginning of training, no other significant differences in conversion efficiency were recorded. Maintenance rations were high in the initial period for all groups, but were lower than the initial values in the second and third periods. While condition factor was significantly lower for the trained groups, there were no differences in percent tissue protein, lipid, or moisture.

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Six foods representing a variety of food products were analyzed by the Assurance Listeria polyclonal enzyme immunoassay (EIA) and by either the Bacteriological Analytical Manual or the U.S. Department of Agriculture culture method for detecting Listeria monocytogenes and related Listeria species. Samples of each food type, at each inoculation level, were analyzed simultaneously by both methods. A total of 19 laboratories representing federal government agencies and private industry in the United States and Canada participated. Food types were inoculated with Listeria species including L. monocytogenes, with the exception of 3 lots of green beans, which were naturally contaminated. During this study, 1764 samples and controls were analyzed and confirmed, of which 492 were positive and 947 were negative by both methods. There were 159 samples that were positive by culture method but negative by the EIA and 188 that were negative by culture method but positive by EIA. Twenty-two samples were negative by EIA and by culture method but confirmed positive when Assurance selective enrichment broths were subcultured to selective agar. The Assurance polyclonal EIA for detecting L. monocytogenes and related Listeria species in foods has been adopted first action by AOAC INTERNATIONAL.