The action of ethylene on the capacity of plant tissues to metabolize cyanide to beta-cyanoalanine was examined. Beta-cyanoalanine synthase (EC 4.4.1.9) catalyzes the reaction between cyanide and cysteine to form beta-cyanoalanine and hydrogen sulfide. Levels of beta-cyanoalanine synthase activity in tissues of 6 day old etiolated pea (Pisum sativum) seedlings were enhanced severalfold by 1 microliter per liter ethylene. The promotive effect of ethylene increased with increasing ethylene concentrations from 0.01 to 100 microliters per liter and with the period of exposure from 3 to 24 hours. Ethylene enhanced beta-cyanoalanine synthase activity in all regions of the seedling (shoots and roots, internodal regions, cotyledons). The promotive effect was eliminated by norbornadiene, a competitive inhibitor of ethylene action. Levels of beta-cyanoalanine synthase in seedlings of four other dicots (Phaseolus aureas, Glycine max, Lactuca sativa, Sinapis arvensis) and two monocots (Hordeum vulgares, Triticum aestivum) were also increased in response to ethylene. Our results suggest an important regulatory role for ethylene in the metabolism of cyanide by higher plants.

Abstract-Trembling aspen (Populus tremuloides Michx.), a common hardwood tree throughout Canada, is being harvested at increasing rates for use in paper and building materials. Piles of aspen logs have been observed to produce a dark, watery, acutely toxic leachate. A laboratory study was undertaken to elucidate the nature, strength, and persistence of aspen leachate toxicity and the chemical composition of the leachate. Leaching from aspen chips in the laboratory was rapid, with 1% mass loss in the first 24 h. Another 2 weeks of immersion was necessary to remove all remaining leachable material (3% total). Fresh aspen leachate derived from a 1:9 wood-water mixture (35 d immersion) was characterized by amber color, low pH (4.0), extremely high BOD (Ͼ2,600 mg/L), and high conductivity (1140 S/cm). The leachate was rich in phenols (30 mg/L), organic carbon (2,480 mg/L), and organic nitrogen (13 mg/ L). Median acutely toxic concentrations of leachate were consistently 1 to 2% of full strength for trout and Daphnia. Inhibition of bacterial metabolism began at concentrations below 0.3%. Leachate was less toxic to plant life but inhibited algal growth at concentrations of 12 to 16%. Toxicity of aspen leachate persisted at the same level as in fresh leachate for more than 2 months unless artificial aeration was provided. Persistence was even greater at low temperature (5ЊC). Aged leachate underwent a transition marked by a rise in pH and dissolved oxygen concentration, a small decline in conductivity, and a color change, from amber to black. Toxicity declined abruptly when the supply of labile toxicants was exhausted, but it sometimes increased again from the products of microbial metabolism. Oxygen depletion, low pH, and phenolic compounds contribute to the toxicity of aspen leachate, but much of the toxic effect must be attributed to other, unidentified constituents.

Trembling aspen (Populus tremuloides Michx.), a common hardwood tree throughout Canada, is being harvested at increasing rates for use in paper and building materials. Piles of aspen logs have been observed to produce a dark, watery, acutely toxic leachate. A laboratory study was undertaken to elucidate the nature, strength, and persistence of aspen leachate toxicity and the chemical composition of the leachate. Leaching from aspen chips in the laboratory was rapid, with 1% mass loss in the first 24 h. Another 2 weeks of immersion was necessary to remove all remaining leachable material (3% total). Fresh aspen leachate derived from a 1:9 wood‐water mixture (35 d immersion) was characterized by amber color, low pH (4.0), extremely high BOD (>2,600 mg/L), and high conductivity (1140 μS/cm). The leachate was rich in phenols (30 mg/L), organic carbon (2,480 mg/L), and organic nitrogen (13 mg/L). Median acutely toxic concentrations of leachate were consistently 1 to 2% of full strength for trout and Daphnia. Inhibition of bacterial metabolism began at concentrations below 0.3%. Leachate was less toxic to plant life but inhibited algal growth at concentrations of 12 to 16%. Toxicity of aspen leachate persisted at the same level as in fresh leachate for more than 2 months unless artificial aeration was provided. Persistence was even greater at low temperature (5°C). Aged leachate underwent a transition marked by a rise in pH and dissolved oxygen concentration, a small decline in conductivity, and a color change, from amber to black. Toxicity declined abruptly when the supply of labile toxicants was exhausted, but it sometimes increased again from the products of microbial metabolism. Oxygen depletion, low pH, and phenolic compounds contribute to the toxicity of aspen leachate, but much of the toxic effect must be attributed to other, unidentified constituents.

No abstract

The regulatory actions of light, temperature, and various nitrogenous compounds on germination of dormant Sinapis arvensis L. seeds were examined to evaluate possible strategies for manipulating germination of soil-bome weed seeds. These treatments, when given singly, had little or no promotive effect on germination. However, KNO3 plus NH4Cl combined with irradiation and a change in temperature to 20 °C after 2 days of incubation at 5 °C induced over 90% germination. The effects of NH2OH∙HCl, KNO2, CS(NH2)2, KCN, and NaN3 were also significantly enhanced when given in combination with a brief exposure to red light and a temperature shift. However, these sources of nitrogen were less effective than KNO3 or NH4Cl in stimulating germination. The promotive effect of light, a temperature shift, and KNO3 and NH4Cl declined when the period of incubation at 5 °C was less than 2 days. This combined treatment was also less effective when the treatment with nitrogen or light was delayed beyond the first 48 h of incubation. Our results indicate that applications of nitrogen fertilizers in combination with surface tillage to expose the seed to light could promote germination of dormant S. arvensis seeds in the field.