The soya seed was treated before sowing with the following biological active substances: Lignohumate B, Lexin, Lexenzym, brassinosteroid, and "Complex treatment" (a mixture of saturated sugar solution, Lexin, fungicide treatment Maxim XL 035 FS and remedial pinolen substance Agrovital). During growing, the influence of biological active substances on root biomass formation and the activity of bacteria for nitrogen fixation was observed. Evaluated parameters were shoot biomass formation and dry mass formation of plants. Harvest values were considered an important output of the whole year soya growth process. As can be observed from the results, the most effective seed treatments were Lexenzym, Lexin, and "Complex treatment", where the yields were high. Moreover, the "Complex treatment" in comparison with the control variant (not treated) improved statistically conclusively not only the final yield but was helpful also for bacteria nodulation and nitrogen fixation (N₂). All biologically active compounds supported the root and shoot biomass formation and the whole plant growth.

In four-year experiments, hop was treated with 7 biologically active substances in two terms during vegetation: Lignohumate max (a mixture of humic acids and fulvic acids), Lexin (a mixture of humic acids and fulvic acids enriched with auxins), Lexenzym (a mixture of humic acids and fulvic acids enriched with auxins, phytohormones and enzymes precursors), Ascophyllum nodosum seaweed extract, synthetic auxin, humic acids and fulvic acids alone. The chlorophyll content was monitored after the application both in the vine leaves and in the branch leaves. After harvesting of the hops from the individual treatments, the yield of dry hops was determined and the cones were analysed for the content of alpha bitter acids. The results show that the most effective hop treatment was the application of Lexin and Lexenzym. The Lexenzym treatment provided a yield of dry hops of 1.86 t/ha, i.e. 0.47 t/ha higher compared with untreated control. The Lexin treatment provided yield higher by 0.41 t/ha of dry hops compared with the untreated control, while the harvested cones contained the most alpha-bitter acids (4.57%).

In 4-year trials, soybean seeds were treated with the following biologically active substances: Lignohumate B (a mixture of humic acids and fulvic acids); Lexin (a mixture of humic acids and fulvic acids enriched with auxins); brassinosteroid (a synthetic analogue of natural epibrassinolide 24) and so-called ‘Complex seed treatment’ (a mixture of a saturated solution of sucrose, Lexin, the fungicide Maxim XL 035 FS and an adjuvant on the basis of pinolene). After harvesting soybean seeds from the individual treatments, they were analysed for oil, protein and fibre contents. The results show that the most effective method was the ‘Complex seed treatment’ which, compared to the untreated variant, significantly increased not only the yield but also the oil content of the seeds.

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