The essential oils from four Arremisiu herbu ulbu populations collected in Israel were investigated for their antibacterial and antispasmodic activities. All the oils had slight antibacterial activities in the concentration range of 1-2 mg/ml. Some correlations between the chemical composition of the oils and their antibacterial activity was observed. All the essential oils tested showed marked antispasmodic effects on rabbit jejunum at about I X~O -~% .The antibacterial together with the antispasmodic effects may explain the extensive use of A. herbu ulbu in folk medicine.
In vivo 31P nuclear magnetic resonance analysis of Escherichia coli cells showed that the intracellular concentration of P, remained constant in wild-type and in a gipT mutant strain whether the cells were grown on excess (2 mM) P1 or sn-glycerol-3-phosphate as a phosphate source. The function of the phoA promoter (measured by 13-galactosidase activity in a phoA-lacZ fusion strain) was repressed when gipT+ cells were utilizing sn-glycerol-3-phosphate as the sole source of phosphate. These cells were devoid of alkaline phosphatase activity. However, the phoA promoter was fully active in a gipT mutant. These results indicated that the repression of the enzyme synthesis was not due to a variation in the level of cytoplasmic Pi but was due to the P, excreted into the periplasm and/or to the medium.
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