Aim: The current experiment was to develop and validate a straight forward RP-HPLC methodology for the determination of Cilnidipine. Methodology: UV spectroscopy was used to estimate Cilnidipine. Action separation of Cilnidipine was achieved by employing a C18 column. Mobile phase combination of methanol: water (90:10 v/v) was tense at the flow of 1 ml/min. Detection was performed at 241 nm. Validation parameters were evaluated in line with the International conference on harmonization (ICH) Q2R1 guidelines. Results: The standardization curve was linear within the varying concentration of 2-10 mg/ml for Cilnidipine with parametric statistic (r2) equal to 0.999. The tactic was found to be accurate (101.66% recovery), precise (intraday, 1.65 and inter day, 1.38) and robust (% RSD was calculated to be 0.66, 0.58 and 0.81 for variation in mobile phase composition, wave length and flow velocity respectively) for the analysis of Cilnidipine. Conclusion: The developed method has passed all the validation tests and can be successfully applied to estimate the presence of Cilnidipine in bulk as well as in pharmaceutical formulations.

The aim of this study was to assess the anti-anxiety and CNS depressant properties of hydro-alcoholic extract of Pongamia pinnata leaves (HALEPP). Standard methods were used to screen the HALEPP's phytochemicals, which showed that the extract contains Flavones, alkaloids, glycosides, proteins and amino acids, gum and mucilage as well as flavonoids, saponins, and steroid hormones. Even at the dose level of 1000 mg/kg body weight, the HALEPP showed no signs of toxicity or mortality in the acute toxicity report. The anti-anxiety behaviour of was studied using light and dark model methods in rats. The CNS depressant action was studied using the extract and actophotometer (Rolex) in rats. The research group (HALEPP 200 gm/kg treated) animals displayed strong anti-anxiety and CNS depressant behaviour similar to the control group (diazepam treated) animals in the current study. The study's findings suggest that the hydro-alcoholic Pongamia pinnata leaf extract has strong anti-anxiety and CNS depressant properties. Keywords: Pongamia pinnata, Anti-Anxiety & CNS depressant