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The usefulness of fullerene modified glassy carbon electrode in mediating the reduction of cefitizoxime in solubilized system has been demonstrated. Due to the unique structure and extraordinary properties, fullerene shows higher catalytic efficiency towards cefitizoxime reduction. The kinetic parameters, electron transfer coefficient (a) and rate constant (K 0 ) across the modified electrode are 0.37 and 0.1081/s respectively. The proposed square-wave voltammetric method is linear over the concentration range 1.2-10.3 mg/mL. The limit of detection (LOD) is found 0.27 ng/mL. High sensitivity and selectivity together with low detection limit of the electrode response make it suitable for the determination of cefitizoxime.