The aim of this experiment was to obtain the differences in protein, moisture and fat content in venison (Mucsulus semimembranosus) of roe deer (Capreolus capreolus L.) and wild boar (Sus scrofa L). The monitoring of wild boar and roe deer was realized from 16 May 2016 to 16 June 2016 in red deer region JXXVIII Tribeč (Western Slovakia, Tribeč mountain). Analysis of basic nutritive components of venison were determined with FoodScan LAB Analyzer (FOSS, Denmark). The obtained average values of experimental traits were as follows: wild boar-23.33%, roe deer-21.55% in protein, respectively 2.46% and 1.8% in fat. The difference in fat content shows significant importance. Moisture was not statistically significant (average: wild boar-73.2%, roe deer-72.9%).