This study aimed to investigate in vitro anticancer activity by antiproliferative activity, antihypertensive activity by angiotensin-converting enzyme inhibition, antidiabetic activity by α-amylase and α-glucosidase inhibitions, and antioxidant activities of camel milk fermented with camel milk probiotic compared with fermented bovine milk. The camel milk probiotic strain Lactococcus lactis KX881782 (Lc.K782) and control Lactobacillus acidophilus DSM9126 (La.DSM) were used to prepare fermented camel and bovine milks separately. The proteolytic activities of water-soluble extract (WSE) in all fermented camel milk were higher than those in fermented bovine milk. The α-glucosidase inhibitions in both milk types fermented by Lc.K782 ranged from 30 to 40%. Camel milk fermented by Lc.K782 had the highest antioxidant activity by 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulphonic acid). The highest angiotensin-converting enzyme inhibition of WSE in camel milk fermented by Lc.K782 was >80%. The proliferations of Caco-2, MCF-7, and HELA cells were more inhibited when treated with WSE of fermented camel milk extracts.
Avian cell lines derived from germinal crescent primordial germ cells and gonadal gonocytes with long-term proliferative capacity in vitro and their subsequent rates of colonization and germline transmission are described. In general, male cultures proliferate more rapidly than female cultures although both can be developed into cell lines of >2 × 10(6) cells, at which time, they can be grown indefinitely and a cell bank can be established. All the cell lines injected into embryos transmitted through the germline with the percentage of germline transmission of both male and female cell lines varying from single digits to the high 90s. The derivation of these primordial germ cell and gonadal cell lines and the subsequent robustness of germline transmission validates these cells as suitable for establishment of lines of chickens bearing novel genetic modifications.
Ginger (Zingiber officinale Rosc.), originated in the Indo-Malayan region, is now widely distributed across the tropics of Asia, Africa, America and Australia. It was domesticated in India and China, which represent the centre of origin of the species. Cultivated ginger though sterile, exhibits variations in rhizome and vegetative characters. The crop is gaining importance as a curative agent for a variety of ailments. Yield and quality traits (such as essential oil, fibre and oleoresin contents) along with volatile and non-volatile constituents are important determinants of the commodity's end product. Cultivar diversity for yield and morphological features is well known in ginger with few primitive types having excellent quality. However, the common name(s) of ginger cultivars are confusing and have resulted in geographical bias in its ex situ conservation. Most of the molecular/biochemical marker studies reported in ginger show low levels of polymorphism in contrast to plentiful phenotypic variability recorded in the species. The large influence of environmental factors on the content of key compounds, lack of seed set and confusion of the common names are the leading constraints to further varietal improvement of ginger. As tailor-made ginger varieties assume future significance these aspects will have relevance.
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