Article Info Received : 09.05.2016 Accepted : 20.07.2016 Contamination of soils with three metals due to the highway and railway transport was studied. Copper was selected as a suitable indicator for both kinds of transportation. Sodium served to assess the level of contamination resulting from the road salting in winter. Mercury was determined in samples taken close to the railway in order to test its release from impregnated wooden ties. All analytes were determined using the methods of the trace element analysis; values are expressed as dry matter. The highest concentrations of copper, sodium and total mercury in soil samples were 52.7 mg/kg, 770 mg/kg and 0.181 mg/kg respectively. The highest copper content was observed in soils taken close to the railroad and the highway. Elevated sodium levels originated from winter road salting -the highest winter value was 770 mg/kg as compared with maximal summer value of 416 mg/kg. The concentration of total mercury in soils depended on the type of railway ties used -the highest values for location with wooden and concrete ties were 0.181 mg/kg and 0.145 mg/kg, respectively. Wooden railroad ties are considered as a potential source of mercury because of impregnation with antifungal mercury compounds.

Risk and essential elements were determined in fruiting bodies of wild growing edible mushrooms Chlorophyllum rhacodes, Suillus grevillei, Imleria badia, and Xerocomellus chrysenteron collected in an unpolluted site in South Bohemia, the Czech Republic. The elements were also determined in underlying soils and the bioconcentration factors were calculated. The analyses revealed that C. rhacodes accumulated Ag, Cu, Rb, Se, Zn, As, Cd, and Tl. On the other hand, S. grevillei accumulated Cd, Rb, Ag, Se, and Cs. I. badia and X. chrysenteron strongly accumulated Rb, Cs, and Ag; these species showed the ability to accumulate Cu and Zn as well. Contents of detrimental Cr VI were in all cases below the quantification limit (0.003 mg kg À 1 dry matter). Studied mushroom species (mainly C. rhacodes) accumulated some toxic elements. However, no considerable effect on human health is expected since they are usually consumed as a delicacy and do not represent a major component of diet.The obtained results revealed that all studied mushroom species contained high levels of Mg. Chlorophyllum rhacodes showed the Mg content of 903 � 53 mg kg À 1 DM. This species contained considerable amounts of Ca (264 � 129 mg kg À 1 ), Zn (127 �

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<i>Pleurotus ostreatus</i> was cultivated on a commercially available wheat straw substrate enriched with Zn and Se. Various amounts of Zn (10, 50, and 100 mg) and Se (1, 5, and 10 mg) in suitable forms ((CH₃COO)₂Zn&#183;2H₂O, Zn(NO₃)₂&#183;6H₂O, and Na₂SeO₃&#183;5H₂O, respectively) were dissolved in 50 ml of deionized water and homogenously nebulized into the substrate block of 2.4 kg weight. The increase in the Zn content in fruiting bodies cultivated on the enriched substrate was relatively low compared with fruiting bodies cultivated on the substrate with no addition at the first flush. The application of different Zn compounds (acetate vs. nitrate) gave similar results. However, the addition of 1 mg of Se into the cultivation substrate block increased the content of Se in fruiting bodies to about 3-6 mg/kg dry matter. This content was one order of magnitude higher compared with the Se content in fruiting bodies harvested from the substrate with no Se addition (&#60; 0.12-0.58 mg/kg dry matter). In the case of the addition of 5 mg of Se, there was a further significant increase in the content of this element to about 40-60 mg/kg dry matter.

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