No abstract

No abstract

Background: High-dose chemotherapy followed by autologous hematopoietic stem cell transplantation (ASCT) is routinely used in various hematologic malignancies. However, dimethylsulfoxide contained in cryopreserved grafts can cause adverse events (AEs). Study design and methods: Forty-three ASCTs were performed with Sepax 2 washed grafts between 7/2016 and 10/2019. The aim of this study was to determine whether washing out dimethyl sulfoxide (DMSO) from transplants using the Sepax 2 (S-100) device is safe and reduces the incidence of DMSO-associated AEs. Results: The washing procedure was automated and that resulted in the satisfactory recovery of total nucleated cells, CD34 + cells, and colony forming units of granulocyte and macrophages (85%, 80%, and 84%, medians). Time to engraftment of leukocytes, granulocytes, and platelets as well as the number of neutropenic days did not differ when compared to 20 consecutive ASCTs without washing. The AE occurrence was lower compared to unwashed grafts: 81% versus 78% during and shortly after grafts administration, 76% versus 69% in the following day. Conclusion:We conclude that the washing of cryopreserved transplants using Sepax 2 was feasible with a high recovery of hematopoietic cells, did not influence time to engraftment, and resulted in the satisfactory reduction of AEs and improved tolerance of the procedure.

Author contributions and disclosures: M.Z. contributed to the research by fluorescence activated cell sorting, DNA processing and amplification, bioinformatic and following data analysis and wrote the manuscript, G. S. designed and led the bioinformatic analysis, T.Š. designed the research, consulted results and wrote the manuscript, V. F. performed the pathway analysis, Z. CH., K. G. and L.B. contributed to bone marrow preparation and DNA processing, T.

No abstract