Objective To investigate the frequency of intestinal parasites in employees of restaurants and cafeterias located in the Commercial Center of São Mateus, Espírito Santo, one of the oldest cities of Brazil. Results The results were positive in 19 % of the samples. The commensal Entamoeba coli (75 %) was the most common followed by Entamoeba histolytica/dispar (37.5 %) and Giardia lamblia (12.5 %). Conclusions Implementing health education measures in the city of São Mateus is suggested by providing permanent health education, as well as conducting parasitological examinations. Such actions will contribute to the improvement of health conditions of the population. Material and Methods